Arabidopsis thaliana Atm3 pET21a+
(Plasmid #172321)

• Purpose: Expresses AtAtm3 in E.coli
• Depositing Lab: Douglas Rees
• Publication: Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi: 10.7554/eLife.76140.

Backbone

• Vector backbone: pET21a(+)
• Backbone size w/o insert (bp): 5340
• Total vector size (bp): 7560
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Arabidopsis thaliana Atm3
• Alt name: AtAtm3
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2220
• GenBank ID: NM_125212.3
• Entrez Gene: ABCB25 (a.k.a. AT5G58270, ABC transporter of the mitochondrion 3, ARABIDOPSIS THALIANA ABC TRANSPORTER OF THE MITOCHONDRION 3, ATATM3, ATM3, ATP-binding cassette B25, HALF-MOLECULE ABC TRANSPORTER ATM3, MCK7.14, MCK7_14, STA1, STARIK 1)
• Tag / Fusion Protein:
  • 6xHis tag (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Ordered from Genscript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry