Addgene

Showing: 1101 - 1120 of 4638 results

Results per page:

Sort results by:

pSH-EFIRES-P-Seipin-miniIAA7-mCherry

Plasmid

#129720

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-mCherry

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
hPAX7-3xHA-IRES-hrGFP (#400)

Plasmid

#78338

Purpose

Overexpression of human PAX7 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter

Depositor

Louis Kunkel, Matthew Alexander

Insert

PAX7 (PAX7 Human)

Use

Tags

3x HA tag and IRES-hrGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pHAGE2-EF1a-MCS-hPOT1_IRES-blast

Plasmid

#166411

Purpose

express hPOT1 in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 (POT1 Human)

Use

Lentiviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

ef-1a

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-mouse c-MycV394D-IRES-RFP

Plasmid

#73573

Purpose

Mammalian retroviral expression of mouse Myc V394D mutant

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

cMyc (Myc Mouse)

Use

Retroviral

Tags

IRES-RFP

Expression

Mammalian

Mutation

V394D

Promoter

MSCV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pRRL CAGpNFlag BAF155 ires GFP

Plasmid

#24561

Depositor

Jerry Crabtree

Article

Ho et al Proc Natl Acad Sci U S A. 2009 Mar 31. 1

Insert

BAF155 (Smarcc1 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

July 16, 2010

Availability

Academic Institutions and Nonprofits only
M78 pIRESpuro-CBP-2xTEV-StrepTag

Plasmid

#17132

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Use

Tags

2xTEV, CBP, and Streptavidin

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-TetR-KRAB-IRES-BLAS

Plasmid

#55820

Purpose

retroviral bicistronic expression vector for TetR-KRAB and Blasticidin S resistance

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Insert

TetR-KRAB

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV eIF4E W73A IRES GFP

Plasmid

#18762

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E W73A (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

W73A

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
hSRF-3xHA-IRES-hrGFP (#479)

Plasmid

#78347

Purpose

Overexpression of human SRF ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter

Depositor

Louis Kunkel, Matthew Alexander

Insert

SRF (SRF Human)

Use

Tags

3x HA tag and IRES-hrGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
Lenti-AcGFP-Sec61-IRES-Blast

Plasmid

#154009

Purpose

Lentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

acGFP-Sec61 (SEC61B Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPyCAG-mKate2-3xNLS-IRES-Pac

Plasmid

#183618

Purpose

Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

Insert

mKate2-3xNLS

Use

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-MinD-IRES-Puro

Plasmid

#213114

Purpose

Expression of untagged MinD in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinD (minD Escherichia coli)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWPI-IRES-eGFP-Mefv iso1

Plasmid

#92104

Purpose

Expression in eukaryotic cells and generating stable cell lines

Depositor

Feng Shao

Article

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

Mefv isoform1 (Mefv Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-Blast 3XFLAG-CHP1

Plasmid

#173166

Purpose

Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1

Depositor

Kivanc Birsoy

Article

Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

Insert

Calcineurin Like EF-Hand Protein 1 (CHP1 Human)

Use

Retroviral

Tags

3XFLAG

Expression

Mutation

Synonymous mutations at sgRNA sites

Promoter

MMLV

Available since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GABRA1(A322D)-IRES2-EGFP

Plasmid

#170821

Purpose

human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

change Alanine 322 to Aspartate

Promoter

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183605

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Use

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHage2-EF1a-mCherryTRF1-IRES-Blast

Plasmid

#182588

Purpose

expresses mcherry-trf1 in mammalian cells

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTRF1 (TERF1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-IRES-hygro-EGFP-Stx17

Plasmid

#174652

Purpose

Vector for overexpression of EGFP-tagged Stx17 with IRES for hygromycin selection.

Depositor

Lukas Kapitein

Article

Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

Insert

EGFP-Stx17 (STX17 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

CMV

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
hSyn-GW-dCas9-SET2-IRES2-mCherry

Plasmid

#202089

Purpose

Neuron-specific expression vector of dCas9-Set2

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2

Use

Tags

3x FLAG and mCherry

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cabp5 (445bp crit)-tdTomato-IRES-AP

Plasmid

#18812

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

Cabp5 (445bp crit)-tdTomato-IRES-AP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only