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Addgene
Showing: 11161 - 11180 of 11515 results
  1. pMMK ENTR 1 CMV mAG beta-catenin

    Plasmid
    #206258
    Purpose
    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mAG Ctnnb1 under the control of CMV promoter.
    Depositor
    Insert
    mAG Ctnnb1 (Ctnnb1 Mouse)
    Use
    Multimate/gateway entr 1
    Tags
    monomeric Azami Green
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pGEMHE-RnCENP-O-11NS-GFP

    Plasmid
    #205181
    Purpose
    Expresses rat CENP-O with 11 mouse-specific point mutations in residues under positive selection; C-term tagged with GFP under T7 promoter - designed for in vitro transcription
    Depositor
    Insert
    CENP-O (Cenpo, Cenpo Rat, Mouse)
    Use
    In vitro transcription
    Tags
    5 glycines linker followed by GFP
    Expression
    Mammalian
    Mutation
    Rat CENP-O with 11 mouse-specific point mutations…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  3. pGEMHE-RnCENP-O-80-GFP

    Plasmid
    #205182
    Purpose
    Expresses chimeric rat CENP-O with mouse CENP-O N-terminus; C-term tagged with GFP under T7 promoter - designed for in vitro transcription
    Depositor
    Insert
    CENP-O (Cenpo, Cenpo Rat, Mouse)
    Use
    In vitro transcription
    Tags
    5 glycines linker followed by GFP
    Expression
    Mammalian
    Mutation
    Chimeric rat CENP-O with mouse CENP-O N terminal …
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  4. pGEMHE-RnCENP-O-203-GFP

    Plasmid
    #205183
    Purpose
    Expresses chimeric rat CENP-O with mouse CENP-O middle region including central RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcription
    Depositor
    Insert
    CENP-O (Cenpo, Cenpo Rat, Mouse)
    Use
    In vitro transcription
    Tags
    5 glycines linker followed by GFP
    Expression
    Mammalian
    Mutation
    Chimeric rat CENP-O with mouse CENP-O middle regi…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  5. pGEMHE-RnCENP-O-298-GFP

    Plasmid
    #205184
    Purpose
    Expresses chimeric rat CENP-O with mouse CENP-O C-terminal RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcription
    Depositor
    Insert
    CENP-O (Cenpo, Cenpo Rat, Mouse)
    Use
    In vitro transcription
    Tags
    5 glycines linker followed by GFP
    Expression
    Mammalian
    Mutation
    Chimeric rat CENP-O with mouse CENP-O C-terminal …
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  6. pGEMHE-MmCENP-O-R298-GFP

    Plasmid
    #205185
    Purpose
    Expresses chimeric mouse CENP-O with rat CENP-O C-terminal RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcription
    Depositor
    Insert
    CENP-O (Cenpo, Cenpo Rat, Mouse)
    Use
    In vitro transcription
    Tags
    5 glycines linker followed by GFP
    Expression
    Mammalian
    Mutation
    Chimeric mouse CENP-O with rat CENP-O C-terminal …
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  7. pCDNA3-MARS-p85(iSH2)

    Plasmid
    #205236
    Purpose
    Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta
    Depositor
    Inserts
    PLEKHA5(143-271)-K163A/R164A (PLEKHA5 Human)
    p85(iSH2) (Pik3r1 Mouse)
    Use
    Tags
    Nuclear Export Sequence and mCherry
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  8. pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby

    Plasmid
    #205280
    Purpose
    Synaptic activity can be imaged with Synaptophysin GCaMP, while simultaneously, a red fluorescence structural marker can be employed to provide a visual reference for the cellular architecture.
    Depositor
    Insert
    GCaMP8s
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA

    Plasmid
    #205281
    Purpose
    CaMP used to measuring calcium changes in neurons, while nls-mRuby is employed to specifically identify cortical GABAergic neurons. This dual labeling approach allows for the simultaneous observation of neuronal activity using GCaMP and the identification of a specific subset of neurons using nls-mRuby, focusing specifically on cortical GABAergic neurons.
    Depositor
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. pAAV_Ef1a_DIO_MyrPalm_mScarlet_WT_IRES_mNeon_minBack

    Plasmid
    #205282
    Purpose
    In order to efficiently visualize projection and cell somata in light-sheet preparations, a combination of membrane-targeted and diffusible red fluorophores was developed. Additionally an ALFA tag
    Depositor
    Insert
    mScarlet
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE

    Plasmid
    #205283
    Purpose
    GCaMP8f fusion to ribosome nanobody to restrict expression to soma (similar to Chen et al. 2020)
    Depositor
    Insert
    GCaMP8f
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 11161 - 11180 of 11515 results