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pAc5.1B-lambdaN-HA-DmGIGYF-C-del331-370-YYMYFWFtoAAAAAAA_AH

Plasmid

#148910

Purpose

Insect Expression of DmGIGYF-del331-370-YYMFWFtoAAAAAA

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-del331-370-YYMFWFtoAAAAAA (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-del331-370_AH

Plasmid

#148911

Purpose

Insect Expression of DmGIGYF-del331-370

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-del331-370 (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-del331-370-YFWFtoAAAA_AH

Plasmid

#148912

Purpose

Insect Expression of DmGIGYF-del331-370-YFWFtoAAAA

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-del331-370-YFWFtoAAAA (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-HA-DmGIGYF-del331-370-YFWFtoAAAA-dsRNAres_AH

Plasmid

#148927

Purpose

Insect Expression of DmGIGYF1-del331-370-YFWFtoAAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF1-del331-370-YFWFtoAAAA-dsRNAres (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PRE-Hsp70BbCas9_1.2

Plasmid

#190796

Purpose

gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.3

Plasmid

#190797

Purpose

aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pACUH-GFP1-10 sec

Plasmid

#172062

Purpose

UAS vector to express GFP1-10 in the ER lumen

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP1-10 sec

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pACUH-GFP1-10 mitochondrial matrix

Plasmid

#172063

Purpose

UAS vector to express GFP1-10 in the mitochondrial matrix

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP1-10 mitochondrial matrix

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCB15

Plasmid

#194756

Purpose

GluRIIA-Rescue-Construct for integration at attP sites

Depositor

Sven Dannhäuser

Article

Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

Insert

GluRIIA (GluRIIA Fly)

Use

Phic31-integration vector

Tags

Expression

Mutation

none

Promoter

Availability

Academic Institutions and Nonprofits only

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pCB19

Plasmid

#194757

Purpose

EGFP-tagged GluRIIA-Construct for integration at attP sites

Depositor

Sven Dannhäuser

Article

Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

Insert

GluRIIA (GluRIIA Fly)

Use

Phic31-integration vector

Tags

EGFP-Tag

Expression

Mutation

none

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCB20

Plasmid

#194758

Purpose

ALFA-tagged GluRIIA-Construct for integration at attP sites

Depositor

Sven Dannhäuser

Article

Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

Insert

GluRIIA (GluRIIA Fly)

Use

Phic31-integration vector

Tags

ALFA-Tag

Expression

Mutation

none

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmImp8_G

Plasmid

#146365

Purpose

Insect Expression of DmImp8

Depositor

Elisa Izaurralde

Insert

DmImp8

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-CG3475_A

Plasmid

#145858

Purpose

Insect Expression of CG3475-3UTR

Depositor

Elisa Izaurralde

Insert

CG3475-3UTR

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
CJ30 (miR-1003 minigene)

Plasmid

#17767

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1003 minigene

Use

Drosophila p-element vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pMT-Flag-RELISH

Plasmid

#63750

Purpose

Expresses drosophila RELISH tagged with a Flag epitopes, under control of metallothionein promoter

Depositor

Cyril Gueydan

Insert

Relish (Rel Fly)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

pMT

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-yki:V5-S168A

Plasmid

#85617

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S168A

Use

Tags

V5

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-yki:V5-S250A

Plasmid

#85618

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S250A

Use

Tags

V5

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST-attB-yki:V5-S111A

Plasmid

#85619

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S111A

Use

Tags

V5

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBS-GGAC-ATGC

Plasmid

#60949

Purpose

Drosophila, backbone for donor plasmid assembly. CRISPR knock-in.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBS-CGGA-GTGC

Plasmid

#60950

Purpose

Drosophila, backbone for donor plasmid assembly. CRISPR knock-in.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge