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Plasmid#89826PurposeFascin promoter for luciferase assayDepositorInsertFascin1 (FSCN1 Human)
UseLuciferaseTagsExpressionMutationPromoterSV40Available sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO.1_puro_shJUND #2
Plasmid#136583PurposeExpresses an inducible short hairpin targeting human JUND sequenceDepositorInsertshJUNDv2 (JUND Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Anti-MMACHC [N230/21R]
Plasmid#114555PurposeMammalian Expression Plasmid of anti-MMACHC (Human). Derived from hybridoma N230/21.DepositorInsertanti-MMACHC (Homo sapiens) recombinant mouse monoclonal antibody (MMACHC Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs PQR>ala
Plasmid#83319Purposehuman DNA-PKcs PQR>alaDepositorInserthuman DNA-PKcs (PRKDC Human)
UseTagsExpressionMammalianMutation5 phosphorylation sites in the PQR cluster substi…PromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs ABCDE>A
Plasmid#83318Purposehuman DNA-PKcs/ABCDE>alaDepositorInserthuman DNA-PKcs (PRKDC Human)
UseTagsExpressionMammalianMutation6 phosphorylation sites in the ABCDE cluster subs…PromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-mTert
Plasmid#162555PurposeOverexpression of TertDepositorInsertOverexpression of Tert (Tert Mouse)
UseTagsExpressionMammalianMutationNonePromoterEF1AAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
IL6R PLAK1
Plasmid#161518PurposeExpresses IL6 receptor (full length) in mammalian cells.DepositorInsertInterleukin 6 Receptor (IL6R Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-EGFP-PARP1
Plasmid#176146PurposeEGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassetteDepositorInsertPoly(ADP-Ribose) Polymerase 1 (PARP1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shCELF4-4405
Plasmid#115466PurposeConstitutive lentiviral expressionDepositorInsertshCELF4-4405 (CELF4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shCELF4-4404
Plasmid#115465PurposeConstitutive lentiviral expressionDepositorInsertshCELF4-4404 (CELF4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHTN_HaloTag_KIF5B[1-560]
Plasmid#213403PurposeTruncated human kinesin-1 with HaloTag at the N-terminusDepositorInsertHaloTag-KIF5B[1-560] (KIF5B Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEEF1D-FLAG (pcDNA 3.1+) LG6-1
Plasmid#37365DepositorInsertEEF1D (EEF1D Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.V1
Plasmid#127847Purposenon-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.V1 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_3xFLAG_V5_ mmDdx58_V5
Plasmid#160108PurposeExpresses murine Ddx58 in mammalian cellsDepositorInsertDdx58 (Rigi Mouse)
UseLentiviralTags3xFLAG, V5ExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX317-MBNL1
Plasmid#115443PurposeConstitutive lentiviral expressionDepositorInsertMBNL1 (MBNL1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRM166 LKB (Luciferase mKate2 Blast)
Plasmid#183502PurposeExpresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.DepositorInsertsFirefly Luciferase
mKate2
BSD
UseLentiviral and LuciferaseTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianMutationPromoterMND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC1-V16-eGFP
Plasmid#122033PurposeAAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and eGFP expressionDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV_Syn_Xph20_eGFP_CCR5TC
Plasmid#187444PurposeEncodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseAAVTagseGFPExpressionMammalianMutationPromoterSynapsinAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-mRuby2-CCR5TC
Plasmid#135531PurposeEncodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseTagsmRuby2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-mEos3.2-CCR5TC
Plasmid#135532PurposeEncodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseTagsmEos3.2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-FLAG-TAOK2
Plasmid#197862PurposeExpression of FLAG-tagged TAOK2 / PSK1 alpha in mammalian cellsDepositorInsertTAOK2 (TAOK2 Human)
UseTagsGFPExpressionMammalianMutationWild TypePromoterSV40Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHTN_HaloTag_KIF5B
Plasmid#213404PurposeFull-length human kinesin-1 with HaloTag at the N-terminusDepositorInsertHaloTag-KIF5B (KIF5B Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-gal4-VP16-gcry-TagBFP
Plasmid#117777PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-P2A-GFP-PGK-Puro
Plasmid#110869PurposeLentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGExpressionMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExp-xMBP-ACE2-CHis
Plasmid#194998PurposeProduction of ACE2 extracellular domainDepositorInsertACE2 (ACE2 Human)
UseTagsHis8 and xMBPExpressionBacterialMutationPromoterT7lacAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Bst LF-6xHis
Plasmid#159148Purposeexpresses His-tagged large fragment of Bst polymeraseDepositorInsertlarge fragment of Geobacillus stearothermophilus DNA polymerase I
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDRM209 LTP (Luciferase tdTomato Puro)
Plasmid#174723PurposeExpresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.DepositorInsertsFirefly Luciferase
tdTomato
pac
UseLentiviral and LuciferaseTagsE2A linker and T2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD WT
Plasmid#169713PurposeBacterial expression of human hnRNPA1-A Low complexity domain including amino acids 186-320DepositorInserthnRNPA1 (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGTag-gal4-VP16-B-actin
Plasmid#117817PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX317-CELF4
Plasmid#115445PurposeConstitutive lentiviral expressionDepositorInsertCELF4 (CELF4 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
GPX8 rescue PLAK1
Plasmid#161517PurposeRescues the expression of GPX8 in GPX8 plenti-CRISPR KO cellsDepositorInsertglutathione peroxidase 8 (GPX8 Human)
UseLentiviralTags3X FlagExpressionMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 TIM9,10
Plasmid#170280PurposeExpresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertUseTagsExpressionBacterialMutationPromoterTim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…Available sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-eGFP-CCR5TC
Plasmid#135530PurposeEncodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseTagseGFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.N
Plasmid#127851Purposenon-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.N VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL403
Plasmid#119953PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
UseTagsExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…PromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-ER Y537S
Plasmid#49499PurposeExpresses HA-tagged ER Y537S mutant in mammalian cellsDepositorInsertESR1 (ESR1 Human)
UseTagsHAExpressionMammalianMutationTyrosine 537 to SerinePromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D
Plasmid#110302PurposeExpresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-IKBalpha-mut (super repressor)
Plasmid#15291DepositorInsertinhibitor of Kappa B alpha (NFKBIA Human)
UseRetroviralTagsExpressionMammalianMutationS32A/S36A: “super-repressor” allele (resistant to…PromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast POLE1
Plasmid#160802PurposeExpress POLE1DepositorInsertPOLE1 (POLE Human)
UseRetroviralTags3xFLAGExpressionMutationshPOLE_1 and shPOLE2 resistantPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-ER D538G
Plasmid#49500PurposeExpresses HA-tagged ER D538G mutant in mammalian cellsDepositorInsertESR1 (ESR1 Human)
UseTagsHAExpressionMammalianMutationAspartic acid 538 to GlycinePromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
-3.6kb human IFN-gamma luc
Plasmid#17599DepositorInsertIFN gamma promoter (IFNG Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation-3.6 kb to +70 of promoterPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
A3Ai-Cas9n-UGI-NLS
Plasmid#109425PurposeExpresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.DepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
UseCRISPRTagsExpressionMammalianMutationInsertion of an L1 intron into the coding sequenc…PromoterCMVAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGTag-eGFP-SV40
Plasmid#117813PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1RA-P2A-GFP-PGK-Puro
Plasmid#110866PurposeLentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertCas9-HF1RA
UseLentiviralTagsFLAGExpressionMutationR661A, Q695A, Q926A, and NLS sequence at the N-te…PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGTag-TagRFP-SV40
Plasmid#117807PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP71-HLA-B0702-His
Plasmid#104850PurposeHLA-B0702 his taggedDepositorInsertmajor histocompatibility complex, class I, B (HLA-B Human)
UseRetroviralTagshisExpressionMammalianMutationPromoterLTRAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-mNeonGreen-CCR5TC
Plasmid#135534PurposeEncodes a specific PSD-95 binder (Xph20) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseTagsmNeonGreenExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRM18 LTN (Luciferase tdTomato Neo)
Plasmid#174721PurposeExpresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.DepositorInsertsFirefly Luciferase
tdTomato
aph
UseLentiviral and LuciferaseTagsE2A linker and P2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX304-ERBB2
Plasmid#175846PurposeExpresses ERBB2DepositorInsertERBB2 (ERBB2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph15-mNeonGreen-CCR5TC
Plasmid#135533PurposeEncodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph15
UseTagsmNeonGreenExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only