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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17599 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL3-basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4800
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIFN gamma promoter
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Alt nameinterferon gamma
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SpeciesH. sapiens (human)
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Insert Size (bp)3670
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Mutation-3.6 kb to +70 of promoter
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Entrez GeneIFNG (a.k.a. IFG, IFI, IMD69)
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Young lab stock #D3299.
SalI should give 8.5kb band
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
-3.6kb human IFN-gamma luc was a gift from Howard Young (Addgene plasmid # 17599 ; http://n2t.net/addgene:17599 ; RRID:Addgene_17599) -
For your References section:
Mucosa-specific targets for regulation of IFN-gamma expression: lamina propria T cells use different cis-elements than peripheral blood T cells to regulate transactivation of IFN-gamma expression. Gonsky R, Deem RL, Bream JH, Lee DH, Young HA, Targan SR. J Immunol. 2000 Feb 1. 164(3):1399-407. PubMed 10640755