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Plasmid#172122PurposeConstitutive expression of EBFP and rtTA-InteinNDepositorInsertEBFP, tTAn-InteinN
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/mArf1(T31N)-HA
Plasmid#79411PurposeExpresses C-terminally HA-tagged Arf1(T31N) in mammalian cellsDepositorInsertArf1 (Arf1 Mouse)
UseTagsHAExpressionMammalianMutationchanged Threonine 31 to AsparaginePromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA
Plasmid#194247PurposeExpresses 3 miRNAs targeting human alpha-SynucleinDepositorInsertmiRaSynuclein(human) (SNCA Human)
UseAAV and RNAiTagsExpressionMutationPromoterCAGAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-JunWT-Myc
Plasmid#47443Purposemammalian expression of WT c-JunDepositorInsertJun (Jun Mouse)
UseTagsFlag and mycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
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LRG2.1-CyOFP1
Plasmid#124771Purpose3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
hV1b-GFP-1
Plasmid#67847Purposemammalian expression of human V1b receptor with GFP tagDepositorInsertV1b receptor (AVPR1B Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pEF1a-tTAc
Plasmid#172123PurposeConstitutive expression of InteinC-tTACDepositorInsertInteinC-tTAc
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cerulean-N1
Plasmid#54742PurposeLocalization: N1 Cloning Vector, Excitation: 433, Emission: 475DepositorTypeEmpty backboneUseTagsCeruleanExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBi2FC-C-GW-P2A:mTagBFP2
Plasmid#216143PurposeBicistronic BiFC vector (Gateway compatible)DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBi2FC-N-GW-P2A:mCherry
Plasmid#216144PurposeBicistronic BiFC vector (Gateway compatible)DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGR595
Plasmid#213785PurposeIntegrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.DepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf6(WT)-HA
Plasmid#79424PurposeExpresses C-terminally HA-tagged Arf6(WT) in mammalian cellsDepositorInsertArf6 (ARF6 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sec61b-V5-TurboID
Plasmid#166971PurposeExpresses ER targeted TurboID for secretome biotinylationDepositorInsertSec61b-V5-TurboID
UseTagsV5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
SBE2-Luc
Plasmid#16500DepositorInsertSmad binding element
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJYDNg
Plasmid#162452PurposeYeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin. The plasmid is carrying the eUnaG2 fluorescent protein and long 2G linker.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsHa and myc and eUnaG2 fluorescent proteinExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
hKCNQ5(WT).ires.GFP-pcDNA3
Plasmid#204360PurposeHeterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)DepositorInsertKCNQ5 (KCNQ5 Human)
UseTagsExpressionMammalianMutationPromoterCMV/T7 RNApolAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC17A9_STOP
Plasmid#161139PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC17A9 (SLC17A9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-TRE-TdTomato
Plasmid#110338PurposeTdTomato reporter vector of iTango and Cal-Light system for AAV productionDepositorInserttetO-TdTomato
UseAAVTagsExpressionMammalianMutationPromotertetOAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-IGK-dAPEX2-KDEL
Plasmid#117183PurposePlasmid name in publication: pAAV-DIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertIGK-dAPEX2-KDEL
UseAAV and Cre/LoxTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf1(WT)-HA
Plasmid#79409PurposeExpresses C-terminally HA-tagged Arf1(WT) in mammalian cellsDepositorInsertArf1 (ARF1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRY2(535, L348F)-mCherry
Plasmid#75372PurposeExpresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherryDepositorInsertCRY2 residues 1-535 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationL348FPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
mc2 mNeon hPGK
Plasmid#206230PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
PPRE-pNL1.3
Plasmid#84394PurposeSecreted Luciferase reporter for PPARgamma activityDepositorInsertPPARG response element (PPARG Human)
UseTagssecNlucExpressionMammalianMutationPromoterPPREAvailable sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF1803
Plasmid#143318PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertUSF1 (USF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40
Plasmid#194690PurposehSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_06-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B5
Plasmid#172846PurposeCRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
CIB81-pmGFP
Plasmid#75366PurposeExpresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on endDepositorInsertCIB1 (CIB1 Mustard Weed)
UseTagsprenylated EGFPExpressionMammalianMutationresidues 1-81 of CIB1PromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40
Plasmid#194691PurposehSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_on-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hRIP3 dRHIM
Plasmid#208788PurposeInducibly expresses human RIP3, lacking RHIM domain, fused to light-activatable Cry2DepositorInsertRIP3 (RIPK3 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationRHIM domain is removedPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
CRY2PHR(W349R)-mCherry
Plasmid#75370PurposeExpresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherryDepositorInsertCRY2 residues 1-498 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationW349RPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEO-CaMKII-TVA-P2A-N2cG
Plasmid#172363PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from excitatory cortical neurons which do not express cre-recombinaseDepositorInsertTVA + B19-N2cG
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgRNA.SFFV.tRFP
Plasmid#169941PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tRFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-mCherry
Plasmid#135189PurposeExpresses TRPML1 with C-terminus mCherry tag in mammalian cells.DepositorInsertTRPML1 (Mcoln1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPING
Plasmid#26046DepositorInsertSS peptide-LacZ-KHHHHHH
UseTagsKHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETGExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJR019
Plasmid#198834PurposeInactivation of G protein subunits through Pertussis toxin (PTX)DepositorInsert15xUAS::PTX-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-LACTB-dAPEX2
Plasmid#117180PurposePlasmid name in publication: pAAV-DIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertLACTB-dAPEX2
UseAAV and Cre/LoxTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2F
Plasmid#15800DepositorInsertUbiquitin conjugating enzyme UbE2F (UBE2F Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3)P Probe
Plasmid#211508PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteriaDepositorInsertFYVE-Hrs x 2 (PIKFYVE Human)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-Tev-Flag-Ube2v2
Plasmid#110070PurposeExpress Halo-Tev-Flag-Ube2v2 (human) in mammalian cellsDepositorInsertHalo-Tev-Flag-Ube2v2 (UBE2V2 Human)
UseTagsHalo-TEV-FlagExpressionMammalianMutationPromoterT7Available sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Luc-Neo
Plasmid#48336PurposeTrasgenically expresses firefly luciferase in T. cruzi with neomycin selectionDepositorInsertfirely luciferase
UseTrypanasoma cruzi expressionTagsExpressionMutationPromoterT. cruzi rRNAAvailable sinceJuly 20, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-Amp_CRISPIE donor B2
Plasmid#172843PurposeCRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mRuby3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_RB1#2
Plasmid#174151PurposeLentiviral vector expressing Cas9 and a sgRNA against the human RB1 geneDepositorInsertRB1 (RB1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUB6-CDC34-HA (pRKB261)
Plasmid#99145PurposeMammalian expression construct to express HA-tagged CDC34DepositorInsertCDC34 (CDC34 Human)
UseTagsHAExpressionMammalianMutationPromoterpTightAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pg-ODD-EGFP
Plasmid#87211PurposeHIF-1a's ODD domain fused to EGFPDepositorInsertHIF-1a ODD domain (HIF1A Human)
UseTagsEGFPExpressionMammalianMutationODD domain (AA 530-652)PromoterCMVAvailable sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf5(WT)-HA
Plasmid#79427PurposeExpresses C-terminally HA-tagged Arf5(WT) in mammalian cellsDepositorInsertArf5 (ARF5 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40
Plasmid#194692PurposehSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_off-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Hygro (656-4)
Plasmid#17446Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, HygroDepositorHas ServiceLentiviral PrepInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP
Plasmid#114369Purposeexpresses Flpo recombinase-dependent GtACR2DepositorInsertGtACR2-EYFP (NEWENTRY )
UseAAVTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
KAT5 sgRNA1
Plasmid#138187Purpose3rd generation lentiviral gRNA plasmid targeting human KAT5DepositorInsertKAT5 (KAT5 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only