-
Plasmid#25874DepositorInsertEYFP H148Q/I152L
UseTagsExpressionMammalianMutationEYFP from pEYFP (Clontech) with H148Q and I152L m…PromoterAvailable sinceAug. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-NLS-TIR1_P2A_NES-TIR1
Plasmid#117699PurposeExpression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNADepositorInsertsTRANSPORT INHIBITOR RESPONSE 1
TRANSPORT INHIBITOR RESPONSE 1
UseTagsHA tag, Myc-NES, and NLSExpressionMammalianMutationcodon optimized for murine expressionPromoterNone, 2A peptide from porcine teschovirus-1 polyp…Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
WT lamin A minigene
Plasmid#20291DepositorInsertlamin A (LMNA Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag WDR5
Plasmid#15552DepositorInsertWdr5 (Wdr5 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-NFIX1
Plasmid#112702PurposeExpression of NFIX1 protein and GFPDepositorInsertNuclear Factor I X1 (Nfix Mouse)
UseTagsGFP and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWPI-N1ICD
Plasmid#185525PurposeConstitutive overexpression of human NOTCH1 intracellular domainDepositorInsertNOTCH1 intracellular domain (NOTCH1 Human)
UseLentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11
Plasmid#193752Purposehuman codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGHDepositorInsertEF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-sfGFP-kanMX (pMaM17)
Plasmid#173452PurposeContains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP-ADH1ter
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9
Plasmid#129391PurposeDerived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.DepositorInsertBurkholderia cenocepacia codon-optimized dCas9
UseCRISPRTagsExpressionBacterialMutationEntire dCas9 codon optimized for the GC-rich B. c…PromoterPrhaBADAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
UseTagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-ChR2-EGFP
Plasmid#114216PurposeExpresses channelrhodopsin-EGFP fusion in CamKIIa+ neuronsDepositorInsertChannelrhodopsin 2
UseAAV and Mouse TargetingTagsEGFPExpressionMammalianMutationPromoterCamKIIa 1.3 kbAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)
Plasmid#163043PurposeQuasAr2-mOrange2 voltage sensor (plasma membrane)DepositorInsertQuasAr2
UseLentiviralTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF1124
Plasmid#143765PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertE2F7 (E2F7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2857
Plasmid#143642PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertID4 (ID4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMLV-EBFP-P2A-Puro
Plasmid#160227PurposeExpresses EBFP in mammalian cellsDepositorInsertEBFP-P2A-Puro
UseRetroviralTagsExpressionBacterial and MammalianMutationPromoterLTRAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-hXRCC1
Plasmid#70759Purposebacterial expression of human XRCC1DepositorInsertXRCC1 (XRCC1 Human)
UseTags6-HISExpressionBacterialMutationPromoterT7/lac operonAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3001
Plasmid#91042PurposeModule A, Promoter: 35S, Gene: GFP, Terminator: HSPDepositorInsertGFP
UseUnspecifiedTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2200
Plasmid#91082PurposeModule C, Promoter: none – to be combined with gRNA array in module B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_B2203), Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRTagsExpressionMutationPromoternone (will be fused to gRNA array in MODULE B)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET41b+_Npl4
Plasmid#117108PurposeProduction of recombinant Npl4 in E.coliDepositorInsertNpl4 (NPLOC4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-tdTomato-cNLS
Plasmid#138532PurposeLentiviral inducible expression of tdTomato with nuclear localization signalDepositorInserttdTomato
UseLentiviralTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE-CMVAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-mPIAS3
Plasmid#15207DepositorInsertPIAS3 (Pias3 Mouse)
UseTagsFlagExpressionMammalianMutationV580IPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-HLA-G SCT-T2A-GFP
Plasmid#205462Purposelentiviral plasmid for expression of HLA-G SCTDepositorInsertHLA-G SCT (HLA-G Human)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
p8092 LentiCRISPR v2 sgNT-1
Plasmid#163315PurposeExpresses Cas9 and a non-targeting control guide RNADepositorInsertsgNT-1
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT G250A
Plasmid#84925Purposeluciferase reporter for mutant TERT promoterDepositorInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationG250APromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tubp-Gal4 in pCaSpeR4
Plasmid#17747DepositorInsertGAL4 (GAL4 Budding Yeast)
UseTagsTubulin 1alpha promoter and hsp70 PolyAExpressionInsectMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE42
Plasmid#90380PurposeGATA3 - Gata3 gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterPPARgAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFlagCMV2-14-3-3sigma
Plasmid#12453DepositorInsert14-3-3 sigma (SFN Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
TFORF0922
Plasmid#142207PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR2F1 (NR2F1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-OC-IRES-BSD
Plasmid#53118PurposeTo create stable cell clones with high-level expression of Cas9 and OCT1DepositorInsertsUseLentiviralTagsIRES-BSD, NLS, and P2AExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9
Plasmid#84261PurposeExpresses AND gate ABA/GA-inducible VPR-Sp dCas9DepositorInsertsGAI-tagBFP-Sp dCas9
PYL1-VPR-p2a-GID1-ABI
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
YFP-RPS5-C1
Plasmid#134534PurposeExpresses YFP-RPS5 fusion proteinDepositorInsertRPS5 (RPS5 Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPIK16045_pX330_Flag-SuperFi-Cas9
Plasmid#184370PurposeMammalian expression of SuperFi-Cas9 nucleaseDepositorInsertSuperFi-Cas9
UseCRISPRTags3X FLAG and NLSExpressionMammalianMutationY1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K…PromoterCBhAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP
Plasmid#215055PurposeExpresses EGFP in mammalian cells from a retroviral vector.DepositorInsertEGFP
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA
Plasmid#194247PurposeExpresses 3 miRNAs targeting human alpha-SynucleinDepositorInsertmiRaSynuclein(human) (SNCA Human)
UseAAV and RNAiTagsExpressionMutationPromoterCAGAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oPent
Plasmid#124664PurposeExpresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coilDepositorInsertSpyCatcher002-oPent
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-Rac1(Q61L)
Plasmid#22783DepositorInsertRac1 (Q61L) (RAC1 A. victoria, Human)
UseTagsCyPetExpressionMammalianMutationRac1 Q61L.PromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSPIKE-P
Plasmid#101172Purposechimeric spike resembling V4 region of prokaryotic 16S rRNA to be used in metagenomicsDepositorInsertV4 16S rRNA chimeric spike
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH51288
Plasmid#50269DepositorInsertpromoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
BCL6-GFP G55A in Artichoke
Plasmid#169935PurposeOverexpression of BCL6-GFP G55A mutant fusionDepositorInsertBCL6 G55A (BCL6 Human)
UseLentiviralTagsGFPExpressionMutation160R>CPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
EHMT1 (2IGQ)
Plasmid#25504PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEHMT1 (EHMT1 Human)
UseTagsHisExpressionBacterialMutationC-terminal domain, residues 951-1235PromoterAvailable sinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits