pCK013
(Plasmid
#105345)
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PurposeBacterial translocation fo proteins into plant cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105345 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBRM-derivate (Szczesny et al., 2010)
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Vector typeBacterial secretion
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Selectable markersChloramphenicol
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Gentamicin, 25 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesecretion signal, ccdB cassette
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SpeciesSynthetic
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MutationBsaI restriction sites removed
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCK013 was a gift from Johannes Stuttmann (Addgene plasmid # 105345 ; http://n2t.net/addgene:105345 ; RRID:Addgene_105345) -
For your References section:
Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system. Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J. PLoS One. 2018 May 30;13(5):e0197185. doi: 10.1371/journal.pone.0197185. eCollection 2018. 10.1371/journal.pone.0197185 PubMed 29847550