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Plasmid#207782PurposeDonor template for mNeon-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780DepositorArticleInsertH3C2 Homology Arms flanking a mNeon-Blast Cassette (H3C2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 CDS A. thaliana MAMI
Plasmid#209186PurposepENTR4 plasmid with CDS of Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI, AT5G54110.1) including stop codon for N-terminal epitope tagsDepositorInsertAT5G54110.1 (MAMI Mustard Weed)
UseGateway-compatible entry vectorTagsExpressionMutationPromoternoneAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMX1463
Plasmid#208805PurposeExpresses plasma-membrane-bound FRB-SBFP2 for rapid relocalization of cytoplasmic proteins tagged with FKBP upon addition of rapamycinDepositorInsertFRB (CRP Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgWAVE2-2
Plasmid#210136Purposeknock out WAVE2 in mammalian cellsDepositorInsertActin-binding protein WASF2 (WASF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgWAVE2-1
Plasmid#210135Purposeknock out WAVE2 in mammalian cellsDepositorInsertActin-binding protein WASF2 (WASF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR
Plasmid#200639PurposeLow STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cellsDepositorInsertSTIM1 (STIM1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENP-O-GFP
Plasmid#205178PurposeExpresses mouse CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorInsertCENP-O (Cenpo Mouse)
UseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationWild type mouse CENP-OPromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-Halo-Tag
Plasmid#196898PurposeExpression of Halo-Tag. Use as control for Halo-tag fusionsDepositorInsertHalo-Tag
UseTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW SYT9 D197, 199N IRES GFP
Plasmid#195701PurposeLentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationfull-length mouse SYT9 with D197, 199N mutationsPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW SYT9 D330, 332N IRES GFP
Plasmid#195702PurposeLentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationfull-length mouse SYT9 with D330, 332N mutationsPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-AcGFP-CI
Plasmid#196849PurposeExpression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoterDepositorInsertAcGFP
UseTagsExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD22y/PfPRMT5-PTP
Plasmid#203160Purposefor tagging PTP to the 5' end of PfPRMT5 gene by single crossoverDepositorInsertPfPRMT5 fragment, PTP tag and pDT 3' UTR
UseTags3' UTR of P. berghei dihydrofolate reductase…ExpressionMammalianMutationPromoterNoAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTdelta5cys
Plasmid#207465PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variantDepositorInsertTdTdelta5cys (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationCys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…PromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shFYN1/GFP4_Seq_B
Plasmid#206135PurposeKnockdown of FYNDepositorInsertFYN shRNA (Fyn Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shFYN1/GFP4_Seq_A
Plasmid#206134PurposeKnockdown of FYNDepositorInsertFYN shRNA (Fyn Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-SAFB-NLS-3XFLAG-V5
Plasmid#196089PurposeExpresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationPromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_SAFBgRNA8
Plasmid#196103PurposeContains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196106, 196107, 196108DepositorInsertSAFB (Safb Mouse)
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_SAFB2gRNA12
Plasmid#196106PurposeContains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196107, 196108DepositorInsertSAFB2 (Safb2 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_SAFB2gRNA13
Plasmid#196107PurposeContains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196108DepositorInsertSAFB2 (Safb2 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
phAG-MCL-CDK5RAP3
Plasmid#204571PurposeExpresses hAG-tagged CDK5RAP3 in mammalian cellsDepositorInsertCDK5RAP3 (AMY2A Human)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only