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pAVPR2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101112

Purpose

Encodes dCas9(N) fused to AVPR2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

AVPR2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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XW21

Plasmid

#65837

Purpose

XW21 Punc-4c-QS-3'UTR-unc-54 (no SL2-mcherry)

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Neurospora crassa OR74A repressor (qa-1S)

Use

Tags

Expression

Worm

Mutation

Promoter

Punc-4c

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFGL1252

Plasmid

#118991

Purpose

A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 68bp MCS (multiple cloning site) which contains 12 unique restriction sites.

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Type

Empty backbone

Use

Agrobacterium tumerfaciens-mediated transformation

Tags

Expression

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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SepOTSγ

Plasmid

#68284

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 1xtRNA-Sep

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pX330A_FokI-1x6

Plasmid

#63606

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCF820_U6-sgRNA-EF1a-mCherry2_lenti

Plasmid

#138316

Purpose

Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SpyCas9 GFP sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pME-mKate2-NS

Plasmid

#109729

Purpose

Multisite gateway vector for 5' tagging with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-Dnm2a

Plasmid

#109571

Purpose

Multisite gateway vector for 3' tagging with Dynamin2a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dynamin2a

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR

Plasmid

#101109

Purpose

Encodes dCas9(N)-DHFR fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)-DHFR

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSθ

Plasmid

#68289

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 2xtRNA-Sep-A37

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSε

Plasmid

#68286

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 1xtRNA-Sep-A37

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAWp7-10

Plasmid

#72742

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pERB174

Plasmid

#58291

Purpose

const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^549-718

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-GFP-FKBPx3
inducible FRB-mCh
FKBP-targeted miRNA
FRB-mCh-Mad1^549-718

Use

Tags

GFP and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAEF6

Plasmid

#136303

Purpose

pAEF5 plasmid with two gRNA inserted

Depositor

Gilles Fischer

Article

Agier et al Methods Mol Biol. 2021;2196:181-198. doi

Insert

double cassette coding for gRNA

Use

CRISPR; Template for pcr to insert double grna co…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHL662

Plasmid

#37636

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

mCherry
GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pK3F-PYK1

Plasmid

#85771

Purpose

N-ICE plasmid pK3F-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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OA959C

Plasmid

#104968

Purpose

Expresses anti-ZIKV transgene in Ae. aegypti

Depositor

Omar Akbari

Article

Buchman et al Proc Natl Acad Sci U S A. 2019 Feb 5. pi

Insert

Anti-Zika Transgene

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pTC151

Plasmid

#70015

Purpose

TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a non-replicating, mutant version of the Gemini Viral Replicon

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (TALENs) + Donor + GVR

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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Degron-KI PIK3CA donor vector

Plasmid

#65621

Purpose

Donor vector to generate Degron-KI DD-PIK3CA

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

PIK3CA Degron-KI left and right HR arms

Use

Tags

Expression

Mutation

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pML3

Plasmid

#108341

Purpose

MIDAS Level-3 destination vector. Used for initiating Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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