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Addgene
Showing: 10881 - 10900 of 13976 results
  1. p5A1-T

    Plasmid
    #92017
    Purpose
    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV protease
    Depositor
    Inserts
    TALE targeting lac operator with 3 TEV cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. p5A1-Ti

    Plasmid
    #92018
    Purpose
    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV protease
    Depositor
    Inserts
    TALE targeting lac operator with 3 TEV cleavage sites
    Catalytically Inactive TEV Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V, C151A
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. p5A3-T

    Plasmid
    #92019
    Purpose
    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Inserts
    TALE targeting lac operator with N-terminal TEV cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. p5A7-T

    Plasmid
    #92020
    Purpose
    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Inserts
    TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. p5B3-T

    Plasmid
    #92021
    Purpose
    Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Inserts
    TALE targeting lysA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. p5C3-T

    Plasmid
    #92022
    Purpose
    Expresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Inserts
    TALE targeting sucA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid
    #92099
    Purpose
    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On 3G Bidirectional
    Availability
    Academic Institutions and Nonprofits only
  8. pCEP4-BaLgp140-TM

    Plasmid
    #111843
    Purpose
    Mammalian expression plasmid for extracellular gp140 from the BaL HIV-1 strain fused to a transmembrane tether
    Depositor
    Insert
    HIV-1 (BaL) Env
    Use
    Tags
    CD5 leader sequence and TM helix from MHC class I
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 10881 - 10900 of 13976 results