Showing: 10861 - 10880 of 13976 results
-
Plasmid#84329PurposeEncodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherryDepositorInsertP2A-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (pGEX2T(TEV))
Plasmid#80630PurposeBacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 1-222PromoterAvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO ALIX Bro1-V (1-702)
Plasmid#80642PurposeResidues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648DepositorInsertALIX Bro1-V (PDCD6IP Human)
UseTagsHis TagExpressionBacterialMutationResidues 1-702PromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N-U6-gRNA TdL
Plasmid#80938PurposeExpresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N-U6-gRNA TdR
Plasmid#80940PurposeExpresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R472A_H477A
Plasmid#83483PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477APromoterAvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A
Plasmid#83484PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).DepositorInsertLbu_C2c2_R1048A_H1053A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR1048A, H1053APromoterAvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A
Plasmid#83485PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A_R1048A_H1053A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477A, R1048A, H1053APromoterAvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode71
Plasmid#83748Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode71
UseRetroviralTagsExpressionMutationdeletion of AA5-233 in GFP (please see depositor …PromoterAvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode81
Plasmid#83758Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode81
UseRetroviralTagsExpressionMutationdeletion of T in starting ATG of GFP (please see …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1R1
Plasmid#97323PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1R1
UseTags6xHis - TEVExpressionBacterialMutationAddition of R residue after T25PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
UseTags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1T1
Plasmid#97327PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T1
UseTags6xHis - TEVExpressionBacterialMutationAddition of T residue after T25PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1
Plasmid#98150PurposeExpresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1
UseTagsHis6-TEVExpressionBacterialMutationWTPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
ZAK (ZAKA-c057)
Plasmid#98225PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertZAKA (MAP3K20 Human)
UseBaculovirusTagsHis-6-TEVExpressionMutationpfam00069:Pkinase 16-257PromoterpolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
DPAGT1 (DPAGT1A-c005)
Plasmid#98228PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDPAGT1A (DPAGT1 Human)
UseBaculovirusTagsHis-6-TEVExpressionMutationpfam00953:Glycos_transf_4 98-275PromoterpolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pJG363
Plasmid#91183PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)DepositorInsertnAtCas9_D10A+gNt_R2+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJG370
Plasmid#91187PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)DepositorInsertnAtCas9_D10A+gRNActrl+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
GFP-CmTMC1truncated
Plasmid#154908PurposeFor the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-CmTMC1truncated
UseTags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-243 and 843-969PromoterAvailabilityAcademic Institutions and Nonprofits only -
GFP-MuTMC2truncated
Plasmid#154909PurposeFor the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-MuTMC2truncated
UseTags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-179 and 691-716PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 10861 - 10880 of 13976 results