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Plasmid#79676Purposemammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutantDepositorInsertPRMT9 (PRMT9 Human)
UseTagsEGFPExpressionMammalianMutationL182A/D183A/I184A/G185A catalytic mutantPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA L5-L4
Plasmid#62138PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPKm- 226
Plasmid#90497PurposepcDNA3 - CMVmin - PIF3 - VPR expresses PIF3 and VPR transactivator domain , under CMV promoterDepositorInsertPIF3-VP64
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
hPlekhm1-C/pIRES puro Glue (S778-A1056)
Plasmid#73456PurposeExpress human C-terminal part (Q778-A1056) Plekhm1 in mammalian cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsCBP tag, HA tag, and strep tagExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 L756A/I757A
Plasmid#19938DepositorInsertcullin 1 L756A/I757A (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationMutation of two adjacent hydrophobic residues, L…PromoterAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
FLAG-OTUB1 T134R,C91S-pcDNA3.1
Plasmid#118212PurposeExpresses FLAG-OTUB1 T134R,C91S in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationchanged cysteine 91 to serine and threonine 134 t…PromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR sgHAL
Plasmid#102315Purposegenetic depletion of HALDepositorInsertsgHAL (HAL Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEG03
Plasmid#73841PurposeEncodes PhyB917 with a C-terminal histidine tag, for protein expression in Dicty.DepositorInsertPhyB(1-917) with C-term his tag (PIL2 Mustard Weed)
UseDictyostelium discoideum expressionTags6x his tagExpressionMutationPromoterAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI-Neo-gp78/2853
Plasmid#11435DepositorInsertgp78 (AMFR Human)
UseTagsExpressionMammalianMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGlomyc-Frat1
Plasmid#124499PurposeExpresses myc-tagged mouse FRAT1 in mammalian cells (N-terminal tag)DepositorInsertFrat1 (Frat1 Mouse)
UseTagsmyc tagExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGlomyc-Frat2
Plasmid#124500PurposeExpresses myc-tagged mouse FRAT2 in mammalian cells (N-terminal tag)DepositorInsertFrat2 (Frat2 Mouse)
UseTagsmyc tagExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
p6595 MSCV-IP-N-HA-GPRC5B
Plasmid#34894DepositorInsertGPRC5B (GPRC5B Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
SGK3-PX (1-126)
Plasmid#119117PurposeBacterial expression of human phox homology (PX) domain, SGK3-PX (1-126)DepositorInsertSGK3-PX (1-126) (SGK3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-shEsr1
Plasmid#120720PurposeLentiviral vector that expresses GFP and an shRNA targeting Esr1 (in pLL3.7).DepositorInsertEsr1 shRNA (Esr1 Mouse)
UseLentiviral and RNAiTagsGFPExpressionMammalianMutationPromoterMouse U6Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Coh-ddFLN4-ELP-HIS-ybbr
Plasmid#153446PurposeE. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc.CohE
UseTags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV tdTomato L5-L2
Plasmid#62152PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and tdTomato module. Compatible with MultiSite Gateway cloningDepositorInserttdTomato
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2xFLAGhYAP2-WW1-mutant
Plasmid#17795DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationTryptophan 199 to Alanine; Proline 202 to AlaninePromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
p2xFLAGhYAP2-WW2-mutant
Plasmid#17796DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationTryptophan 258 to Alanine Proline 261 to AlaninePromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAM5187
Plasmid#120086PurposeCyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid.DepositorInsertpAM4787 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPK-351
Plasmid#157921PurposepcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBDDepositorInsertsPIF3-MTAD
IRES
PhyB(1-621)-SV40NLS-Gal4DBD
UseSynthetic Biology; OptogeneticsTagsHA tag and SV40NLSExpressionMammalianMutationpif3 1-523PromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only