pLV-shEsr1
(Plasmid
#120720)
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PurposeLentiviral vector that expresses GFP and an shRNA targeting Esr1 (in pLL3.7).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120720 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLL3.7
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Backbone manufacturerMIT
- Backbone size w/o insert (bp) 7649
- Total vector size (bp) 7700
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Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEsr1 shRNA
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Alt nameEstrogen receptor alpha, ERa
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gRNA/shRNA sequenceGGCATGGAGCATCTCTACA
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SpeciesM. musculus (mouse)
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Entrez GeneEsr1 (a.k.a. ER, ER-alpha, ERa, ERalpha, ESR, Estr, Estra, Nr3a1)
- Promoter Mouse U6
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Tag
/ Fusion Protein
- GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer U6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The 19 nucleotide targeting sequence for Esr1 was obtained from this publication:
https://www.ncbi.nlm.nih.gov/pubmed/16803960
This plasmid is also featured in the following article: https://pubmed.ncbi.nlm.nih.gov/32482639
(J Neurosci. 2020 )
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-shEsr1 was a gift from Amy Lasek (Addgene plasmid # 120720 ; http://n2t.net/addgene:120720 ; RRID:Addgene_120720) -
For your References section:
Estrogen Receptor beta in the Nucleus Accumbens Regulates the Rewarding Properties of Cocaine in Female Mice. Satta R, Certa B, He D, Lasek AW. Int J Neuropsychopharmacol. 2018 Apr 1;21(4):382-392. doi: 10.1093/ijnp/pyx118. 10.1093/ijnp/pyx118 PubMed 29294029