Showing: 10781 - 10800 of 13976 results
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Plasmid#131338PurposegRNA to delete a nucleation site near HoxD. Use with HoxD del us1DepositorInsertEVX2-gRNA-C-del
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-st1CAS9-pA
Plasmid#131542PurposeExpresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized st1Cas9
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-CjCAS9-pA
Plasmid#131543PurposeExpresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCjCas9
UseCRISPRTagsFLAG-SV40NLS, SV40NLS, and polyAExpressionMammalianMutationHuman codon-optimizedPromoterCAG, T3AvailabilityAcademic Institutions and Nonprofits only -
Rap1A FLARE
Plasmid#134926PurposeRap1A biosensorDepositorInsertRap1A, mCerulean3, RaIGDS RBD, YPet
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Rap1B FLARE
Plasmid#134927PurposeRap1B biosensorDepositorInsertRaIGDS RBD, YPet, Cerulean, Rap1B
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-Munc18-1wt
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP2
Plasmid#135551PurposeExpress mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP2
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP3
Plasmid#135552PurposeExpress rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP3
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP8
Plasmid#135553PurposeExpress mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP8
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18b
Plasmid#135554PurposeExpress rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.DepositorInsertMunc18b
UseTags6xHis tag and TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18c
Plasmid#135555PurposeExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.DepositorInsertMunc18c
UseTags6xHis tag and TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-RABIF
Plasmid#135556PurposeExpress human RABIF in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertRABIF
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Rab10wt
Plasmid#135557PurposeExpress mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.DepositorInsertRab10
UseTags6xHis tag, TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-intron-BaLgp160
Plasmid#100918PurposeMammalian expression plasmid for Env from the BaL HIV-1 isolateDepositorInsertHIV-1 (BaL) Env with upstream intron
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-Q769gp160
Plasmid#100928PurposeMammalian expression plasmid for Env from the Q769.d22 HIV-1 strainDepositorInsertHIV-1 (Q769.d22) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-Q842gp160
Plasmid#100931PurposeMammalian expression plasmid for Env from the Q842.d12 HIV-1 strainDepositorInsertHIV-1 (Q842.d12) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002 DA-MBP
Plasmid#102832PurposeBacterial expression of unreactive version of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002 DA-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationReactive aspartic acid has been mutated to an ala…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3 EcoRI Methylase-AmilCP
Plasmid#85166PurposeFor methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.DepositorInsertEcoRI Methylase-AmilCP
UseTagsAmilCPExpressionBacterialMutationPromoterJ23100 promoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 10781 - 10800 of 13976 results