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Plasmid#149347PurposeMammalian expression vector for SpyCas9 and puromycin resistanceDepositorInsertSpyCas9
UseCRISPRTagsFlag tagExpressionMammalianMutationPromoterCbhAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA PIK3C2G KD1
Plasmid#162008PurposePIK3C2gamma shRNADepositorInsertPIK3C2G (PIK3C2G Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA SNX9 KD1
Plasmid#162014PurposeSNX9 shRNADepositorInsertSNX9 (SNX9 Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA SNX9 KD2
Plasmid#162015PurposeSNX9 shRNADepositorInsertSNX9 (SNX9 Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA PIK3C2A KD5
Plasmid#162006PurposePIK3C2alpha shRNADepositorInsertPIK3C2A (PIK3C2A Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA PTEN KD4
Plasmid#162010PurposePTEN shRNADepositorInsertPTEN (PTEN Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA INPP5E KD2
Plasmid#162005PurposeINPP5E shRNADepositorInsertINPP5E (INPP5E Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato. PGK-puro
Plasmid#136264PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomatoExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-cjSTELLA-mCerulean-PuroTK
Plasmid#141029PurposeReporter integrationDepositorInsertmCerulean
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mouse 3' Ubf AID GFP PuroR
Plasmid#127901PurposeHDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Ubf GeneDepositorInsertUbf (Ubtf Mustard Weed, Mouse)
UseDonor plasmidTagsGFP, AID, PuroRExpressionMutationInserting GFP AID 2A Puro into mouse 3' UbfPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mouse 3' Hp1a AID GFP PuroR
Plasmid#127897PurposeHDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a GeneDepositorInsertHp1a (Cbx5 Mustard Weed, Mouse)
UseDonor plasmidTagsGFP, AID, PuroRExpressionMutationInserting GFP AID 2A Puro into mouse 3' Hp1aPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v4)-PGK-Puro-BFP
Plasmid#117143PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v4 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v5)-PGK-Puro-BFP
Plasmid#117144PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v5 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP
Plasmid#117140PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v1 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v2)-PGK-Puro-BFP
Plasmid#117141PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v2 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti C-MYC-DDK Puro PNRC1
Plasmid#123300PurposeMammalian expression of PNRC1 C-MYC-FLAGDepositorInsertPNRC1 (PNRC1 Human)
UseLentiviralTagsMYC-FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-d80
Plasmid#114025PurposeInducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell linesDepositorInserteGFP-d80-HEC1 (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 1 to 80 deletedPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-AuroraB L154A/H250Y
Plasmid#108489Purposeexpression of vsv-AuroraB Analog sensitive (L154A/H250Y)DepositorInsertAuroraB (AURKB Human)
UseTagsVSVExpressionMammalianMutationAnalog sensitive L154A/H250YPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV(shRNA)-Puro-6 CCEPR Vb2
Plasmid#108278PurposeshRNA for CCEPR lncRNADepositorInsertCCEPR (CCEPR Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824E-S828E
Plasmid#91999PurposeExpresses FLAG-HA-AGO2 (S824E-S828E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824E, S828EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824E-S831E
Plasmid#92000PurposeExpresses FLAG-HA-AGO2 (S824E-S831E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824E, S831EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S828E-S831E
Plasmid#92001PurposeExpresses FLAG-HA-AGO2 (S828E-S831E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS828E, S831EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824A-T830A
Plasmid#92003PurposeExpresses FLAG-HA-AGO2 (S824A-T830A)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824A, T830APromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup88-XbaI
Plasmid#87328PurposeTo express shRNA against human Nup88DepositorInsertshRNA against human Nup88
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup93-XhoI
Plasmid#87330PurposeTo express shRNA against human Nup93DepositorInsertshRNA against human Nup93
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Mustela putorius furo
Plasmid#78116PurposeNt His-tag Mustela putorius furo Sacsin 94-1381DepositorInsertSacsin Mustela putorius furo 94-1381
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SIRPT1-Mustela putorius furo
Plasmid#75333PurposeGST fusion Mustela putorius furo Sacsin 94-1381DepositorInsertSacsin Mustela putorius furo 94-1381
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro p21 K161Q, K163Q
Plasmid#78792PurposeRetroviral expression of p21 K161Q, K163QDepositorInsertP21 (CDKN1A Human)
UseRetroviralTagsExpressionMammalianMutationK161Q, K163Q muationPromoter5'LTRAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-RIPK3 2KR(89,501)
Plasmid#78832Purposeretroviral expression of RIPK3 2KR(89,501)DepositorInsertRIPK3 (RIPK3 Human)
UseRetroviralTagsExpressionMammalianMutationK89R, K501RPromoter5'LTRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-RIPK3 2KR(55,363)
Plasmid#78831Purposeretroviral expression of RIPK3 2KR(55,363)DepositorInsertRIPK3 (RIPK3 Human)
UseRetroviralTagsExpressionMammalianMutationK55R, K363RPromoter5'LTRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro p21 K161R, K163R
Plasmid#78791PurposeRetroviral expression of p21 K161R, K163RDepositorInsertp21 (CDKN1A Human)
UseRetroviralTagsExpressionMammalianMutationK161R, K163R_muationPromoter5'LTRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV/TO-Puro-FLAG-hDIXDC1 WT
Plasmid#61218PurposeLentivirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterCMV/TOAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Delta-ATG1-mMCL1
Plasmid#45823PurposeThis plasmid was made to show that the two MCL-1 bands DO NOT arise from a non-canonical translation initiation downstream of the start codon.DepositorInsertDelta ATG1-mMCL1
UseTagsExpressionMammalianMutationThe first amino acid (ATG) is deleted from the fu…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-mMCL1 Delta C22
Plasmid#45820DepositorInsertmMCL-1 Delta C22
UseTagsExpressionMammalianMutationC-terminal 22 amino acids are deleted from full l…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Delta N67-mMCL1
Plasmid#45819DepositorInsertDelta N67-mMCL-1
UseTagsExpressionMammalianMutationN-terminus 67 amino acids deleted from full lengt…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-THAP11-rescue-3xFLAG
Plasmid#36998DepositorInsertTHAP11 (THAP11 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationMutations were generated at codons 273-275 conver…PromoterAvailable sinceMay 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
integrin beta6 Y762F pBabe puro
Plasmid#13597DepositorInsertintegrin beta 6 Y762F (ITGB6 Human)
UseRetroviralTagsExpressionMammalianMutationY762FPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE
Plasmid#85452PurposeLentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.DepositorInsertU6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai
Plasmid#202571PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Methionine 91 to Alanine, ORF1p Glu…PromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai
Plasmid#202568PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…PromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai
Plasmid#202569PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Arginine 261 to AlaninePromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cas9-T2A-EGFP-ires-puro
Plasmid#78311PurposeExpresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.DepositorInsertWT SpCas9-T2A-EGFP-ires-puromycin resistance
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterCAGAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin
Plasmid#134860PurposeExpresses EGFP-tagged recycling endosome markerDepositorInsertRAB11B (RAB11B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato-NLS. PGK-puro
Plasmid#136261PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomato-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A iRFP670 P2A puro
Plasmid#122182PurposeThe plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsUseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-dCas9-VPR-wpA-pPuroTK
Plasmid#214127PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-Halo-hMRE11(DB1Δ/DB2Δ)
Plasmid#208395PurposeTo generate stable cell line expressing Halo-human MRE11(DB1Δ/DB2Δ) by Retroviral infection.DepositorInsertHuman MRE11 (MRE11 Human)
UseLuciferase and RetroviralTagsExpressionMammalianMutationDB1delta/DB2deltaPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-P2A-GFP-PGK-Puro
Plasmid#110869PurposeLentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGExpressionMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only