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Showing: 1061 - 1080 of 2137 results

  2. G protein alpha-s(alpha3beta5)

    Plasmid
    #55799
    Purpose
    This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T in alpha-s sequ…
    Promoter
    CMV
    Available since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    55799-plasmid-map-sequence-id-119340

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  5. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177434
    Purpose
    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5r4 Rat)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177434-plasmid-map-sequence-id-348465

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  10. pHAGE2-RSV-jGCaMP7s-W

    Plasmid
    #126690
    Purpose
    Mammalian calcium sensor protein.
    Depositor
    Darrell Kotton
    Insert
    jGCaMP7s
    Use
    Lentiviral
    Tags
    6xHIS, T7, Xpress
    Expression
    Mutation
    A52V, A317L
    Promoter
    RSV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126690-plasmid-map-sequence-id-245673

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  11. mEos2-PSD95-N-14

    Plasmid
    #57411
    Purpose
    Localization: Membrane Scaffold, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    PSD95
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pLenLox_U6 Nlgn3

    Plasmid
    #59359
    Purpose
    Lentiviral expression vector: Neuroligin 3 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.
    Depositor
    Peter Scheiffele
    Article
    Chih et al Science. 2005 Feb 25;307(5713):1324-8. E
    Inserts
    Neuroligin 3 shRNA (rat) (Nlgn3 Rat)
    eGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and mouse U6
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    59359-plasmid-map-sequence-id-92032

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  15. pQZ210

    Plasmid
    #106414
    Purpose
    Co-expresses trans SNARE complex with pDC183 in E.coli
    Depositor
    Axel Brunger
    Article
    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d
    Inserts
    Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
    Syntaxin-1A fragment 191-256 (Stx1a Rat, Synthetic)
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Fragment 191-256 , Codon optimization and Fragmen…
    Promoter
    T7
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106414-plasmid-map-sequence-id-204167

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  16. mVenus-Lysosomes-M-20

    Plasmid
    #56618
    Purpose
    Localization: Lysosome Membrane, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    LAMP1 M
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    R8 silent mutation; CGT
    Promoter
    CMV
    Available since
    Dec. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    56618-plasmid-map-sequence-id-241076

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  18. KCC2-pHext

    Plasmid
    #104076
    Purpose
    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)
    Depositor
    Igor Medyna
    Article
    Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00
    Insert
    KCC2 (b isoform) (Slc12a5 Rat)
    Use
    Tags
    pHluorine tag inserted in the second putative tra…
    Expression
    Mammalian
    Mutation
    EcoR1 site in rat KCC2 was removed by silent muta…
    Promoter
    UbC
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104076-plasmid-map-sequence-id-199360

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  20. EGFP-Cx43-7

    Plasmid
    #56498
    Purpose
    Localization: Gap Junctions, Excitation: 493, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Cx43
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1061 - 1080 of 2137 results