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Plasmid#223478Purposesrx-105 fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertsrx-105 (srx-105 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY137
Plasmid#223484Purposetax-2 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInserttax-2 (tax-2 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY138
Plasmid#223485Purposetol-1(3k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInserttol-1(3k) (tol-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY144
Plasmid#223491Purposeunc-39(3k) fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertunc-39 (3k) (unc-39 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSX2719
Plasmid#200763PurposeExpresses mCherry fused MS2 coat protein and 24xGCN4 (Suntag) with the tissue-specific promoter semo-1 in C. elegans.DepositorInsertMCP-24xGCN4
UseTagsExpressionWormMutationPromotersemo-1Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSX2720
Plasmid#200764PurposeExpresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans.DepositorInsertscFv-sfGFP
UseTagsExpressionWormMutationPromotercol-19Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSX2739
Plasmid#200765PurposeExpresses mKate2-tag fused CDC-42 with 8xMS2(V1) stem loops in C. elegans, and the tissue-specific promoter col-19 is used.DepositorInsert8xMS2
UseTagsmKate2ExpressionWormMutationPromotercol-19Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pHMTc-FBF-2 (121-632)
Plasmid#218664PurposeExpression of MBP:6xHIS:FBF-2(121-632)DepositorInsertfbf-2 (fbf-2 Nematode)
UseTagsMBP-6xHISExpressionBacterialMutationamino acids 121-632 onlyPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry]
Plasmid#197599PurposePan-neuronal expression of SNG-1::CRY2(D387A)olig(535) in neurons of C. elegans. CRY2(D387A) is photoinactiveDepositorUseTagsExpressionWormMutationD387A, E490GPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV18 [punc-47::SNG-1::CRY2olig(535)]
Plasmid#197598PurposeExpression of SNG-1::CRY2olig(535) in GABAergic motor neurons of C. elegansDepositorUseTagsExpressionWormMutationE490GPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV12 [psng-1::SNG-1::CRY2olig(535)]
Plasmid#197596PurposePan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegansDepositorUseTagsExpressionWormMutationE490GPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
RT-5
Plasmid#220442PurposeConstruct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)DepositorInsertPUF3 (puf-3 Nematode)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS178
Plasmid#215678PurposeEmpty repair plasmid for ChrII split hygromycinR landing padDepositorInsert5'HA + MCS + loxP + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK2097
Plasmid#218665PurposeExpression of MBP:6xHIS:FBF-2(Y479A, 121-632)DepositorInsertfbf-2 (fbf-2 Nematode)
UseTagsMBP-6xHISExpressionBacterialMutationamino acids 121-632 only, with Y479A mutationPromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4203
Plasmid#200160PurposePrig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFPDepositorInserttwk-40 (twk-40 Nematode)
UseTagsGFPExpressionWormMutationPromoterPrig-3Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH4293
Plasmid#200161PurposePtwk-40s GCaMP6s::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 mNeptuneDepositorInsertGCaMP6
UseTagsmNeptuneExpressionWormMutationPromoterPtwk-40Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH4316
Plasmid#200171PurposePflp-18 LoxP EBFP (stop) LoxP twk-40(gf) GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40(gf) GFPDepositorInserttwk-40 (twk-40 Nematode)
UseTagsGFPExpressionWormMutationPromoterPflp-18Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH4506
Plasmid#200255PurposePnpr-4 TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFPDepositorInsertTeTx
UseTagsmCherryExpressionWormMutationPromoterPnpr-4Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4508
Plasmid#200256PurposePflp-18 LoxP EBFP LoxP TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFPDepositorInsertTeTx
UseTagsmCherryExpressionWormMutationPromoterPflp-18Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only