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We narrowed to 13,976 results for: LEA

Showing: 10761 - 10780 of 13976 results

Results per page:

pcDNA3-blueCKAR(T/A)

Plasmid

#118473

Purpose

Negative-control mutant for blueCKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueCKAR(T/A)

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-RAB-EKARev

Plasmid

#118474

Purpose

ddRFP-based single-color biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-RAB-EKARev(T/A)

Plasmid

#118475

Purpose

Negative-control mutant for RAB-EKARev biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev(T/A)

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-RAB-AKARev

Plasmid

#118478

Purpose

ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-AKARev

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-RAB-AKARev(T/A)

Plasmid

#118479

Purpose

Negative-control mutant for RAB-AKARev biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-AKARev(T/A)

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-His6-Ubc9-C93A

Plasmid

#133910

Purpose

Bacterial expression of His6-Ubc9-C93A

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9

Use

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 93 to Alanine

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-His6-Ubc9-C138A

Plasmid

#133911

Purpose

Bacterial expression of His6-Ubc9-C138A

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9

Use

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 138 to Alanine

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS-foxc1a-2a-nlstdTomato

Plasmid

#132970

Purpose

full-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Inserts

tdTomato
foxc1a (foxc1a Zebrafish)

Use

Tags

NLS and myc

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
BII-gR-PnGW

Plasmid

#133355

Purpose

Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Use

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Availability

Academic Institutions and Nonprofits only

Enlarge
MLKS2del4ec

Plasmid

#130857

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Expression

Mutation

Deletion of terminal four amino acids

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MLKS2delKASHec

Plasmid

#130858

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Expression

Mutation

eGFP-FLAG-HA

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MLKS2delARMec

Plasmid

#130860

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Expression

Mutation

Deletion of armadillo domain

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MLKS2del4exp

Plasmid

#130861

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of terminal four amino acids

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MLKS2delKASHexp

Plasmid

#130862

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of KASH domain

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MLKS2delTMexp

Plasmid

#130863

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of transmembrane domain

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MLKS2delARMexp

Plasmid

#130864

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of armadillo domain

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SKOR1-gRNA-N-del

Plasmid

#131331

Purpose

gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-C-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

SKOR1-gRNA-N-del

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SKOR1-gRNA-C-del

Plasmid

#131332

Purpose

gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-N-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

SKOR1-gRNA-C-del

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
LMX1B-gRNA-N-del

Plasmid

#131333

Purpose

gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

LMX1B-gRNA-N-del

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
HoxD del us1

Plasmid

#131337

Purpose

gRNA to delete a nucleation site near HoxD. Use with HoxD del ds1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EVX2-gRNA-N-del

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge