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Showing: 10741 - 10760 of 13976 results

  5. p667-UBC-DYRK3-V5-miniTurbo_IDG-K

    Plasmid
    #158190
    Purpose
    Gateway destination clone of DYRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    DYRK3-V5-miniTurbo (DYRK3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    R293H- please see depositor comments
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    158190-plasmid-map-sequence-id-328919

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  7. GFP-CmTMC1truncated

    Plasmid
    #154908
    Purpose
    For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.
    Depositor
    Motoyuki Hattori
    Article
    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d
    Insert
    GFP-CmTMC1truncated
    Use
    Tags
    8histidine-EGFP
    Expression
    Insect
    Mutation
    Truncations of the residues 1-243 and 843-969
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    154908-plasmid-map-sequence-id-310626

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  8. GFP-MuTMC2truncated

    Plasmid
    #154909
    Purpose
    For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.
    Depositor
    Motoyuki Hattori
    Article
    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d
    Insert
    GFP-MuTMC2truncated
    Use
    Tags
    8histidine-EGFP
    Expression
    Insect
    Mutation
    Truncations of the residues 1-179 and 691-716
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    154909-plasmid-map-sequence-id-306870

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  11. pJK503

    Plasmid
    #155380
    Purpose
    dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    155380-plasmid-map-sequence-id-309009

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  12. pJK511

    Plasmid
    #155384
    Purpose
    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    155384-plasmid-map-sequence-id-309004

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  13. pJK520

    Plasmid
    #155386
    Purpose
    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    155386-plasmid-map-sequence-id-309003

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  14. pJK526

    Plasmid
    #155387
    Purpose
    PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA4 in dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    155387-plasmid-map-sequence-id-309008

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  15. pJK528

    Plasmid
    #155388
    Purpose
    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    155388-plasmid-map-sequence-id-324484

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  17. pcDNA3_HLA-cmyc-GRM3 C127A

    Plasmid
    #140451
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    140451-plasmid-map-sequence-id-291874

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  18. Antares-SEPLuc

    Plasmid
    #141086
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-EMCV IRES-SEPLuc
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 66 to 91 on the Luciferas…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    141086-plasmid-map-sequence-id-296862

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  19. pGBW-m4046810

    Plasmid
    #149251
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Availability
    Industry, Academic Institutions, and Nonprofits
    149251-plasmid-map-sequence-id-357431

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  20. pGBW-m4046940

    Plasmid
    #149261
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Availability
    Industry, Academic Institutions, and Nonprofits
    149261-plasmid-map-sequence-id-296392

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Showing: 10741 - 10760 of 13976 results