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alpha actinin delta ABD-GFP

Plasmid

#66935

Purpose

Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

Depositor

Pere Roca-Cusachs, Michael Sheetz

Article

Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Alpha-actinin 1 (ACTN1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

actin binding domain (amino acids 30–253) is dele…

Promoter

CMV

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
VV201: ChR2(C128S)-eGFP in fubi

Plasmid

#58510

Purpose

Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

ChR2(C128S)-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Cysteine 128 to Serine

Promoter

Ubiquitin

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL2-deltaTM

Plasmid

#109021

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-deltaTM (ATL2 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

truncated form without C-terminal ER transmembran…

Promoter

EIF-1a promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-PLlacO1-deGFP-T500

Plasmid

#45394

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

deGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PL-lacO1

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MyD88-CpLxIS

Plasmid

#131348

Purpose

Expresses MyD88 with 2 copies of pLxIS motif from STING fused to its C-terminus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88-CpLxIS (Myd88 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-mouse-Caspase-11

Plasmid

#183358

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-11 (Casp4 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-ArchT-GFP (PV2527)

Plasmid

#99039

Purpose

AAV-mediated expression of ArchT under the CamKII promoter

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

ArchT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinA GFP

Plasmid

#32119

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

GFP and Kar2

Expression

Yeast

Mutation

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
FKBP-epsin(144-575)-GFP

Plasmid

#100733

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

epn1 (Epn1 Mouse)

Use

Tags

FKBP and GFP

Expression

Mammalian

Mutation

clathrin binding middle part aa 144-575

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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114_pAAV-ProC22-CatCh-GFP-WPRE

Plasmid

#125957

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC22

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag GFP 4E-BP1 Rheb15

Plasmid

#112761

Purpose

express GFP-4EBP1-Rheb15

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)

Use

Lentiviral

Tags

Flag eGFP and Rheb15

Expression

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFPC1/GgVcl 1-851 A50I mutant

Plasmid

#46269

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-851 A50I mutation (VCL Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

1-851 (Vh) head domain, A50I mutation

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-myosin Vb tail 1436-1846

Plasmid

#162714

Purpose

Mammalian expression of rat WT Myosin Vb with deletion of the 1435 N-terminal residues

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

Myosin Vb (Myo5b Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT with deletion of the 1435 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2-msfGFP

Plasmid

#174492

Purpose

bacterial expression of human SEPT2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

SEPTIN2 (SEPTIN2 Human)

Use

Tags

His6-TEV and msfGFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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R26-EGFP MMEJ donor vector

Plasmid

#137926

Purpose

R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-EGFP-bpA with micro-homology arms

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMP71-mtGFP-llo-lama4-alg8

Plasmid

#174605

Purpose

expresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 and Lama4

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

palmytoylGFP w/C-term fusion LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 Lama4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Snap25-LSL-2A-GFP targeting vector

Plasmid

#61575

Purpose

Target a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Snap25-LSL-2A-GFP

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL3-deltaTM

Plasmid

#109025

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-deltaTM (ATL3 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

truncated form without C-terminal ER transmembran…

Promoter

EIF-1a promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSF143-wArchon2-KGC-EGFP-ER2

Plasmid

#108430

Purpose

Codon-optimized Archon2 fluorescent voltage reporter in C.elegans expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

wArchon2-KGC-EGFP-ER2

Use

Tags

Expression

Worm

Mutation

Promoter

flp-18

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-smURFP-P2A-GFP

Plasmid

#197151

Purpose

Expresses the protein of smURFP-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

smURFP-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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