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Showing: 10661 - 10680 of 13976 results

  8. His-NavMs

    Plasmid
    #100004
    Purpose
    Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP
    Depositor
    Paul DeCaen
    Article
    Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10
    Insert
    His-NavMs, Voltage gated sodium channel
    Use
    Tags
    Hexa-His tag followed by a thrombin cleavage site
    Expression
    Mammalian
    Mutation
    C-terminus from a codon-optimized synthetic gene,…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    100004-plasmid-map-sequence-id-192452

    Enlarge

  9. pMSP1D1R1

    Plasmid
    #97323
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1R1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of R residue after T25
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    97323-plasmid-map-sequence-id-182662

    Enlarge

  10. pMSP1D1G1

    Plasmid
    #97325
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1G1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of G residue after T25
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    97325-plasmid-map-sequence-id-182623

    Enlarge

  11. pMSP1D1T1

    Plasmid
    #97327
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1T1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of T residue after T25
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    97327-plasmid-map-sequence-id-182625

    Enlarge

  13. ZAK (ZAKA-c057)

    Plasmid
    #98225
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ZAKA (MAP3K20 Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Expression
    Mutation
    pfam00069:Pkinase 16-257
    Promoter
    polyhedrin
    Availability
    Academic Institutions and Nonprofits only
    98225-plasmid-map-sequence-id-193304

    Enlarge

  14. DPAGT1 (DPAGT1A-c005)

    Plasmid
    #98228
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DPAGT1A (DPAGT1 Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Expression
    Mutation
    pfam00953:Glycos_transf_4 98-275
    Promoter
    polyhedrin
    Availability
    Academic Institutions and Nonprofits only
    98228-plasmid-map-sequence-id-193399

    Enlarge

  18. pEG593

    Plasmid
    #40119
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (ATG through 648 bp of 3'UTR) R274E K318E (RNAi res) (mex-5 Nematode)
    Use
    Gateway destination vector
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    R274E, K318E, Exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Availability
    Academic Institutions and Nonprofits only

  19. pEG628

    Plasmid
    #40120
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (aa1-244) (RNAi resistant) (mex-5 Nematode)
    Use
    Gateway destination vector
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    contains aa1-244, Exon 2 recoded to be RNAi-resis…
    Promoter
    4.4 kb mex-5 promoter fragment
    Availability
    Academic Institutions and Nonprofits only

  20. pEG610

    Plasmid
    #40195
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    contains aa 1-355; exon 2 recoded to be RNAi-resi…
    Promoter
    4.4 kb mex-5 promoter fragment
    Availability
    Academic Institutions and Nonprofits only
Showing: 10661 - 10680 of 13976 results