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Plasmid#80490PurposeGateway Destination donor vector for AAVS1 targeting (puromycin selection)DepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCI-neo FlucSM EGFP
Plasmid#90171PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0101
Plasmid#90998PurposeModule A, Promoter: 35S, Gene: AtCas9, Terminator: HSPDepositorInsertAtCas9
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE-VEGFA EF1a-rtTA3
Plasmid#195881PurposeLentiviral construct of doxycycline inducible VEGFADepositorInsertVEGFA (Vegfa Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2_hMGFP-60A
Plasmid#200393PurposeA plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertmonster GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE EF1α dCas9-KRAB
Plasmid#50919PurposeConstitutive dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840APromoterEF1alphaAvailable sinceMarch 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
HERV-Kcon-RT for Split-PE (pJUL3120)
Plasmid#190107PurposeExpresses HERV-Kcon RT (human codon optimization)DepositorInsertHERV-Kcon-RT
UseTagsbpNLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc
Plasmid#117728PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc geneDepositorInsertNanoLuc
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ153
Plasmid#69304PurposeGateway entry vector with pco-dCas9-3X(SRDX)DepositorInsertpco-dCas9-3X(SRDX) (Plant codon-optimized)
UseCRISPRTags2XFLAGExpressionMutationSRDX (X3) repressor fusion; NLS fusionPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP
Plasmid#63215PurposeExpression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationPromoterCMV-EF1α hybrid (CEF)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCE059-SiT-Cas12a-[Activ]
Plasmid#128136PurposeExpress both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcriptDepositorInsertAsCas12a-[Activ]-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH2.30_Gag-MCP-dpol-T2A-eGFP
Plasmid#205538PurposeExpress HIV Gag-MCP-dpol-T2A-eGFPDepositorInsertHIV Gag-MCP-dpol
UseTagsExpressionMammalianMutationpol frameshift sequence recodedPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT137-splitC
Plasmid#122605Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI118-SiT-Cas12a-[Ind]
Plasmid#128406PurposeInducible expression of both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDF0387 pcmv-hudiscas7-11-r978-gggs-r1294
Plasmid#186984Purposemammalian expression of cas7-11-r978-gggs-r1294DepositorInsertcas7-11-r978-gggs-r1294
UseTagsExpressionMammalianMutationr978-gggs-r1294 deletionPromoterAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT100.106b
Plasmid#122601Purposeclone APOBEC1 phage by SapI Golden GateDepositorInsertAPOBEC1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miniGFP1-P2A-FusionRed
Plasmid#188732PurposeExpress miniGFP1 and FusionRed in mammalian cellsDepositorInsertminiGFP1
UseAAVTagsFusionRedExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9 VP64-T2A-GFP
Plasmid#53192PurposeCo-expresses human optimized S. pyogenes dCas9-VP64 and GFPDepositorInserthumanized dead Cas9 VP64 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sar1b-YFP
Plasmid#166897Purposemammalian expression of Sar1b tagged with YFPDepositorInsertSar1b
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_smFP FLAG
Plasmid#59756Purposeencodes multiple copies of an epitope tag FLAGDepositorInsertsmFP _FLAG
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only