Showing: 10641 - 10660 of 13976 results
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Plasmid#21699DepositorInsertProtein G domain B1 fused N-terminal zinc binding domain of human papillomavirus type 16 (residues 2-74) (E6 Human papillomavirus type 16)
UseTagsProtein G B1 domainExpressionBacterialMutationGB1 (1-56aa) fusion tag with mutations at three p…PromoterAvailabilityAcademic Institutions and Nonprofits only -
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myc-Rictor
Plasmid#1860PurposePlease see Addgene item #11367 for an updated version of this plasmid.DepositorInsertRictor (RICTOR Human)
UseTagsMycExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMSP1E3D1
Plasmid#20066PurposeBacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formationDepositorInsertMSP1E3D1
UseTags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeats of …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pBP-T7_RBS-His6-Thrombin
Plasmid#72979PurposeT7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)DepositorInsertT7+RBS+His6+thrombin
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-hDcp2
Plasmid#72213Purposemammalian expression of Flag-tagged human Dcp2DepositorInsertDcp2 (DCP2 Human)
UseTagsFlagExpressionMammalianMutationQ170R (please see depositor's comment below)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-hDcp2
Plasmid#72216Purposebacterial expression of GST-tagged human Dcp2DepositorInsertDcp2 (DCP2 Human)
UseTagsGSTExpressionBacterialMutationF146Y and E198K (please see depositor's comm…PromotertacAvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-S727A-STAT3
Plasmid#71446PurposeRetroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationS727APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-K49R-STAT3
Plasmid#71447PurposeRetroviral expression of K49R-STAT3. Please note that this plasmid contains K49R-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationK49RPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-K140R-STAT3
Plasmid#71448PurposeRetroviral expression of K140R-STAT3. Please note that this plasmid contains K140R-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationK140RPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-K685R-STAT3
Plasmid#71449PurposeRetroviral expression of K685R-STAT3. Please note that this plasmid contains K685R-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationK685RPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-WT-STAT3
Plasmid#71450PurposeRetroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 NFKB1
Plasmid#67126PurposeStandard for cell-free expression benchmarking.DepositorInsertNFKB1 (NFKB1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
MSP2262
Plasmid#70703PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A & H557A in SaCas9PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
MSP2279
Plasmid#70706PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A and H557A in SaCas9PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCMV-bArrestin2-TevC-P2A-TdTomato
Plasmid#89869Purposeexpresses iTango bArrestin2-TevC component and TdTomato bicistronicallyDepositorInsertbArrestin2-TevC-P2A-TdTomato
UseSynthetic BiologyTagsExpressionMammalianMutationR173H in TdTomato (please see depositor comments …PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCMV-NES-CRY2PHR-TevC
Plasmid#89877Purposeexpresses NES-CRY2PHR-TevC of BLITz systemDepositorInsertNES-CRY2PHR-TevC
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
His-MBP-NRBF2 (1-159)
Plasmid#99331PurposeMammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complexDepositorInsertNuclear receptor-binding factor 2 (NRBF2 Human)
UseTags6xHis-MBP-TEVExpressionBacterialMutationAA1-159 are presentPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pYPQ127B
Plasmid#99883PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoterDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNCS mOrange
Plasmid#91764PurposeBacterial expression of a fluorescent protein, monomeric Orange (mOrange)DepositorInsertmonomeric Orange
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 10641 - 10660 of 13976 results