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pcDNA3.1-MUC2 D1D2D' with cleavable His tag

Plasmid

#187993

Purpose

Expresses MUC2 D1D2D' assembly with cleavable His tag in mammalian cells

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Mucin2 D1D2D' (MUC2 Human)

Use

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pRVdGL-1EGFP

Plasmid

#98036

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-hRAD18 dSAP

Plasmid

#68830

Purpose

Expresses c-myc-tagged human RAD18 deleting SAP domain

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 deleting SAP domain (RAD18 Human)

Use

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
myc-hRAD18 dC2

Plasmid

#68831

Purpose

Expresses c-myc-tagged human RAD18 deleting Polymerase eta binding domain

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 deleting Polymerase eta binding domain (RAD18 Human)

Use

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pAdTrack: shRev-Erbalpha

Plasmid

#22749

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

shRev-Erbalpha (Nr1d1 Mouse)

Use

Adenoviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2010

Availability

Academic Institutions and Nonprofits only
MLS-mir-17-92-Mut92

Plasmid

#64094

Purpose

expresses a mutated version of mir-17-92 (mutation of miR-92 seed)

Depositor

Lin He

Article

Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

Insert

miR-17-92 (MIR17HG Human)

Use

Tags

Expression

Mammalian

Mutation

mutation of miR-92 seed sequence

Promoter

MSCV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
pRVdG-4Cre

Plasmid

#98034

Purpose

First-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

mCre

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pICE-EGFP-FLAG-Ku70siR-Mut6E

Plasmid

#46962

Purpose

For constitutive or doxycycline-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

Ku70 (XRCC6 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Mut6E corresponding to K282E K287E T300E K331E K3…

Promoter

CMV Tet

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-miR-34b/34c

Plasmid

#63933

Purpose

MSCV vector for overexpressing mouse miR-34b/34c

Depositor

Lin He

Article

Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

Insert

microRNA 34b/34c (Mir34c, Mir34b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
pAdCMV/V5-DEST-Y705F-STAT3-3xFlag

Plasmid

#99261

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3 (Y705F)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - Y705F (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Expression

Mutation

Y705F (contains a silent mutation: nucleotide 271…

Promoter

CMV

Available since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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MO70-N-Flag-hCRACR2A-isoform-a

Plasmid

#79592

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pShuttle-EGFP-CLASP2 340-1084

Plasmid

#24383

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1084) (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant that retains MT lattice bi…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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MAP4K3-GFP K45E

Plasmid

#21734

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 8. ()

Insert

MAP4K3 (MAP4K3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Mutation of the ATP-binding site (K45E). This ver…

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-CLASP2 512-650

Plasmid

#24409

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (512-650) (CLASP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant with only MT plus end trac…

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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BII-ChiNW-IGF2

Plasmid

#133383

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

IGF2 (IGF2 Human)

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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myc-hRAD18 d6BD

Plasmid

#68832

Purpose

Expresses c-myc-tagged human RAD18 deleting hRAD6 binding domain

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 deleting hRAD6 binding domain (RAD18 Human)

Use

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
pCGN-BCL-6Δ (pCGN-BCL6-delta)

Plasmid

#40338

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

BCL-6Δ (BCL6 Human)

Use

Tags

Expression

Mammalian

Mutation

Missing four of six zinc fingers (see note below)…

Promoter

CMV

Available since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only
pLPC-Myc-TRFcT

Plasmid

#44573

Depositor

Eros Lazzerini Denchi

Article

Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

Insert

chimeric TRF (TERF2, TERF1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

TRF2 where TRFH domain (aa42-241) has been replac…

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only
pTOPO-MCL1S159A

Plasmid

#21606

Depositor

Ulrich Maurer

Article

Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

Insert

MCL-1 S159A (MCL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Serine 159 mutated to Alanine, abolishing phosph…

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-DC-mutant-G435R

Plasmid

#64684

Purpose

expresses single mutated WRAP53beta at G435R, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutation at G435R

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only