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pDEST- IP2.0(codon for C.elegans)

Plasmid

#109216

Purpose

IP2.0 for C. elegans (Codon optimization and introns).

Depositor

Takeshi Ishihara

Article

Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

Insert

IP2.0 (Inverse Pericam 2.0)

Use

Tags

His-tag

Expression

Worm

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pMXs-IG

Plasmid

#51258

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

3xFLAG-dCas9

Use

CRISPR and Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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Arabidopsis thaliana Atm3 d80 pET21a+

Plasmid

#173045

Purpose

Expresses AtAtm3 in E.coli

Depositor

Douglas Rees

Article

Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

Insert

Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

with N-terminal 80 amino acids deletion

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTarget TMEM97-P2A-mCardinal

Plasmid

#157747

Purpose

Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

Depositor

Andrew Kruse

Article

Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

Insert

TMEM97 (TMEM97 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV14_NOP58

Plasmid

#73063

Purpose

expression clone for human NOP58 with C-terminal 3xFLAG-tag

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

NOP58 ribonucleoprotein (NOP58 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLX-Z4 (GB3375)

Plasmid

#160648

Purpose

pLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)

Depositor

Fabio Pasin, Juan Antonio García

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB3052(gRNA X-4, XI-3, XII-5)

Plasmid

#73294

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase validation vector_SCP1_empty

Plasmid

#99299

Purpose

Luciferase validation vector; empty vector without insert to determine baseline

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Luciferase; Starr-seq luciferase validation vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSc-humanADAR2

Plasmid

#171106

Purpose

Expresses human ADAR2 in yeast.

Depositor

Brenda Bass

Article

Macbeth et al Methods Enzymol. 2007;424:319-31. doi: 1

Insert

human ADAR2 (ADARB1 Human)

Use

Tags

His12_TEV

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3-WHaloCaMP1a

Plasmid

#205312

Purpose

Expression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-gp78c/2767

Plasmid

#11429

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78c (AMFR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

n/a

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pLdCH

Plasmid

#84291

Purpose

Express gRNA and Cas9 in Leishmania with Hygromycin resistance

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Tags

Expression

Mutation

Promoter

L. donovani ribosome RNA promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

Plasmid

#100279

Purpose

In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

Depositor

Yi Zhang

Article

Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

Insert

Kdm6b (Kdm6b Mouse)

Use

Tags

Flag epitope tag

Expression

Mammalian

Mutation

Mutated catalytic domain (1025aa-End) (please see…

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTET2 (N1041)

Plasmid

#89737

Purpose

Expresses the N1041 truncation to mouse TET2 in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

TET2 (Tet2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

N-term 1041 truncation of mouse TET2 (contains fi…

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBLO ddd64.1

Plasmid

#123121

Purpose

E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX

Use

CRISPR

Tags

Expression

Mutation

Mutated amino acids in catalytic triad to alanine…

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTet-O-NEUROG3-T2A-PuroR

Plasmid

#162341

Purpose

Lentiviral expression of NEUROG3 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

NEUROG3 (NEUROG3 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pPGS dnTcf-4(deltaN41)

Plasmid

#19284

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

Tcf-4 (TCF4 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Dominant negative, lacks N-terminal 41 amino acids

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

Plasmid

#199368

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOstar-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pQTEV-ANP32A

Plasmid

#31563

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pBS GDF10

Plasmid

#19186

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Growth differentiation factor 10 precursor (GDF10 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only