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pLIX403-ccdB-EBFP2

Plasmid

#158562

Purpose

Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)

Plasmid

#191389

Purpose

Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus

Depositor

Yannick Doyon

Insert

hCdt1(1-100)

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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hSLBP_mNG_FS_Donor

Plasmid

#191387

Purpose

Donor for C-terminal tagging of endogenous hSLBP

Depositor

Yannick Doyon

Insert

mNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus (SLBP Human, Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLIX403-ccdB-EYFP

Plasmid

#158564

Purpose

Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLIX403-ccdB-NatMx

Plasmid

#158563

Purpose

Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRR2

Plasmid

#166097

Purpose

Gal inducible expression of dCas9-FokI

Depositor

Rebecca Burgess

Insert

dCas9-FokI

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9-D10A, H840A

Promoter

Gal-inducible

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pET32a-LIC

Plasmid

#62310

Purpose

E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Use

Tags

6xHis, S-tag, and Trx-Tag

Expression

Bacterial

Mutation

Promoter

T7-lacO

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIC81

Plasmid

#63065

Purpose

Mammalian expression of EGFP-TEV-S-tag PMFI for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Insert

PMFI, IMAGE clone 6148250 (PMF1 Human)

Use

Tags

EYFP and TEV-S

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pITI1

Plasmid

#166094

Purpose

Gal inducible expression of dCas9-FokI

Depositor

Rebecca Burgess

Insert

dCas9-FokI

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9-D10A, H840A

Promoter

Gal-inducible

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pREP4xN_ccdB

Plasmid

#79022

Purpose

Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection

Depositor

Göran Akusjärvi, Pernilla Bjerling

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pREP3xN_ccdB

Plasmid

#79021

Purpose

Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selection

Depositor

Göran Akusjärvi, Pernilla Bjerling

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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T4 Lysozyme T21C K124C WT*

Plasmid

#18243

Depositor

Brian Matthews

Insert

T4 Lysozyme (e Bacteriophage T4)

Use

Tags

Expression

Bacterial

Mutation

T21C K124C WT*

Promoter

Available since

Aug. 26, 2008

Availability

Academic Institutions and Nonprofits only
pREP82xN_ccdB

Plasmid

#79026

Purpose

Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection

Depositor

Göran Akusjärvi, Pernilla Bjerling

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pREP81xN_ccdB

Plasmid

#79025

Purpose

Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selection

Depositor

Göran Akusjärvi, Pernilla Bjerling

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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T7m

Plasmid

#121030

Purpose

MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

S. pyogenes tracrRNA terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pREP41xN_ccdB

Plasmid

#79023

Purpose

Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection

Depositor

Göran Akusjärvi, Pernilla Bjerling

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pREP42xN_ccdB

Plasmid

#79024

Purpose

Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection

Depositor

Göran Akusjärvi, Pernilla Bjerling

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-miRFP670nano3

Plasmid

#184664

Purpose

Monomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCas9-Blast

Plasmid

#52962

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Has Service

Lentiviral Prep

Inserts

Cas9
Blasticidin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

May 8, 2014

Availability

Academic Institutions and Nonprofits only

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pAG1159 TOM20-ECFP-FIREmate

Plasmid

#196693

Purpose

Expresses TOM20-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

TOM20-ECFP-FIREmate

Use

Tags

FIREmate

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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