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Plasmid#196973PurposeBacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tagDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTagsGFP and GSTExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1_GST-SNAP-DmKHC[1-421]
Plasmid#196976PurposeBacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GSTDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTagsGST and SNAPExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
K365-iLid
Plasmid#188456PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
UseTags6xHis and iLidExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
K365-micro
Plasmid#188457PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
UseTags6xHis-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHACK-QF2-AgU6-mCherry
Plasmid#184530PurposeHACK-compatible plasmid for generating T2A-QF2 knockins in Anopheles mosquitoes with a mCherry selectable marker.DepositorInsertQF2
UseCRISPRTagsExpressionMutationPromoterAnopheles U6 promoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALuWBB
Plasmid#183202PurposedsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNADepositorInsertw+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
UseCRISPR; Recombines at attp landing site in fly ge…TagsExpressionInsectMutationPromoterU6-3Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF_1-640_AH
Plasmid#148933PurposeInsect Expression of DmGIGYF1_1-640DepositorInsertDmGIGYF1_1-640 (Gyf Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF_331-374-GST-V5His6_AH
Plasmid#148934PurposeInsect Expression of DmGIGYF_331-374DepositorInsertDmGIGYF_331-374 (Gyf Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF_641-1574_AH
Plasmid#148936PurposeInsect Expression of DmGIGYF1_641-1574DepositorInsertDmGIGYF1_641-1574 (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-F361AF367A_AH
Plasmid#148937PurposeInsect Expression of DmGIGYF-F361AF367ADepositorInsertDmGIGYF-F361AF367A (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-W349A_AH
Plasmid#148938PurposeInsect Expression of DmGIGYF-W349ADepositorInsertDmGIGYF-W349A (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-WFFtoAAA_AH
Plasmid#148939PurposeInsect Expression of DmGIGYF-WFFtoAAADepositorInsertDmGIGYF-WFFtoAAA (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-Y58AY60AM65A_AH
Plasmid#148940PurposeInsect Expression of DmGIGYF1-Y58AY60AM65ADepositorInsertDmGIGYF1-Y58AY60AM65A (Gyf Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-YYMYFWFtoAAAAAAA_AH
Plasmid#148941PurposeInsect Expression of DmGIGYF1-YYMFWFtoAAAAAAADepositorInsertDmGIGYF1-YYMFWFtoAAAAAAA (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-PPFPPFtoAAAAAA_AH
Plasmid#148908PurposeInsect Expression of DmHPat-PPFPPFtoAAAAAADepositorInsertDmHPat-PPFPPFtoAAAAAA (Patr-1 Fly)
UseTagsExpressionInsectMutationthree silent mutations compared to the sequence g…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4EHP-W114A_AH
Plasmid#148909PurposeInsect Expression of Dm4EHP-W114ADepositorInsertDm4EHP-W114A (4EHP Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-C-del331-370-YYMYFWFtoAAAAAAA_AH
Plasmid#148910PurposeInsect Expression of DmGIGYF-del331-370-YYMFWFtoAAAAAADepositorInsertDmGIGYF-del331-370-YYMFWFtoAAAAAA (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-del331-370_AH
Plasmid#148911PurposeInsect Expression of DmGIGYF-del331-370DepositorInsertDmGIGYF-del331-370 (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-del331-370-YFWFtoAAAA_AH
Plasmid#148912PurposeInsect Expression of DmGIGYF-del331-370-YFWFtoAAAADepositorInsertDmGIGYF-del331-370-YFWFtoAAAA (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-YFWFtoAAAA_AH
Plasmid#148913PurposeInsect Expression of DmGIGYF-YFWFtoAAAADepositorInsertDmGIGYF-YFWFtoAAAA (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only