Showing: 10561 - 10580 of 13976 results
-
Plasmid#168211PurposeMammalian expression of nuclear-localized dTomato with 3 high affinity MS2 loopsDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pMEH294 NLS dTomato 1x high affinity MS2 loop
Plasmid#168213PurposeMammalian expression of nuclear-localized dTomato with 1 high affinty MS2 loopDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pMEH295 NLS dTomato 1x wild type MS2 loop
Plasmid#168214PurposeMammalian expression of nuclear-localized dTomato with 1 wild type MS2 loopDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pMEH303 NLS dTomato 6x high affinity MS2 loops
Plasmid#168215PurposeMammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loopsDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDGE1109
Plasmid#202773PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDGE1177
Plasmid#202775PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCB11
Plasmid#201111PurposepSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.DepositorInsertsCcaR
tetA(C)
sfGFP
UseTagsExpressionBacterialMutationC-to-T transition at pos. no. 1049 of tetA(C) lea…PromoterJ23100 and PcpcG2-172AvailabilityAcademic Institutions and Nonprofits only -
Clomeleon
Plasmid#203723PurposeA genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentrationDepositorInsertcyan fluorescent protein linked yellow florescent protein
UseTagsExpressionBacterial and MammalianMutationCyan Fluorescent Protein, Topaz Fluorescent Prote…PromoterAvailabilityAcademic Institutions and Nonprofits only -
PB-NES-ZapCV2-Zinc dead
Plasmid#203850PurposeExpressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cellsDepositorInsertZapCV2-Ala zinc dead sensor
UseTagsNuclear Export SignalExpressionMammalianMutationCys and His in Zap1 is mutated with AlaPromoterCMV promoterAvailabilityAcademic Institutions and Nonprofits only -
pEF HaloTag-SYB2
Plasmid#197282PurposeEncodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseTagsExpressionMammalianMutationQ76V and F77WPromoterEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro2-H2B-iRFP
Plasmid#197352PurposeEncoding nuclear-targeted iRFP713.DepositorInsertiRFP with nucleus-targeting sequence
UseLentiviralTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterEF-1αAvailabilityAcademic Institutions and Nonprofits only -
pET21b-hHNF4aORF-1
Plasmid#206959PurposeThe ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.DepositorInsertHNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
H5_fold-0_Elsa
Plasmid#201825PurposeExpresses H5_fold-0_Elsa in bacterial cellsDepositorInsertH5_fold-0_Elsa
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA32 (MSW#497)
Plasmid#208068PurposeExpression of GFP-tagged hsRPA2 in human cellsDepositorInsertRPA2 (RPA2 Human)
UseTagsGFPExpressionMammalianMutationT88I- please see depositor commentsPromoterAvailabilityAcademic Institutions and Nonprofits only -
MARS-nGFP
Plasmid#205234PurposeExpresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cellsDepositorInsertPLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
UseTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
2xMARS-nGFP
Plasmid#205235PurposeExpresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cellsDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-2xMARS-PLD
Plasmid#205237PurposeExpresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMFDepositorInsertsUseTagsNuclear Export Sequence and mCherryExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET28b_RanWT
Plasmid#186270PurposeExpression vector of His-RanQ69L in E.coliDepositorInsertGTP-binding nuclear protein Ran
UseTagsHis taggedExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pMCSG53-DsbC
Plasmid#186623PurposeE. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.DepositorTypeEmpty backboneUseTagsDsbC-6His-TEVExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lyn11-STf
Plasmid#182008PurposeCMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell linesDepositorInsertLyn11-STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 10561 - 10580 of 13976 results