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Plasmid#64854PurposeEndoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-GCaMP3
UseTagsN-terminal 27 amino acids from CYP450ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_253
Plasmid#91125PurposeTransformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NADP-Snifit-cyto
Plasmid#113917PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorInsertNADP-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDisplay sHRPb-KDEL
Plasmid#73150PurposeSmall fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.DepositorInsertsHRPb-KDEL
UseTagsV5 tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TBG-Cyto-TurboID
Plasmid#149414PurposeHepatocyte-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertCyto-TurboID
UseAAVTagsV5ExpressionMutationPromoterTBGAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Omega1
Plasmid#165864PurposeOmega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP11
Plasmid#215559PurposepKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 originDepositorInsertsCas9
gRNA targeting pBR322 origin of replication
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23105 and tetAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
tUI
Plasmid#122661PurposeExpress tUI protein in E. coliDepositorUseTagsHis-tagExpressionMutationPromoterT7 promoterAvailable sinceJuly 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-C1-GFP-SF3B6-FLAG
Plasmid#86870PurposeTo express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.DepositorInsertSF3B6 (SF3B6 Human)
UseTagsEGFP and FLAGExpressionMammalianMutationnonePromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM146
Plasmid#127218PurposeBeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDSDepositorInsertLuc, BBM, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLG018 (AVAST type 2)
Plasmid#157896PurposeExpresses the AVAST type 2 defense systemDepositorInsertAVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-DeltaFosB
Plasmid#68545PurposeAAV expression of DeltaFosBDepositorInsertDeltaFosB-IRES-GFP
UseAAVTagsExpressionMammalianMutationtruncated splice variant of FosBPromoterCMVAvailable sinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCGB
Plasmid#135283PurposeGBPart - CGB Luciferase CDSDepositorInsertClick Beetle Green Luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ca-FLARE (TF)
Plasmid#92213PurposeTranscription factor component of FLAREDepositorInsertNRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16
UseAAVTagsExpressionMammalianMutationPromotersynapsinAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo10
Plasmid#203961PurposeCloning plasmid containing the erythromycin resistance gene insertDepositorInsertErythromycin Resistance Gene
UseTagsExpressionPlantMutationPromoterN/AAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDAS12395_PEAR-GFP_2.0-preedited
Plasmid#177185PurposePEAR-GFP3 control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRT802
Plasmid#68113PurposeIntegration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.DepositorInsertsaac3(IV)
aph11
oriT
attP
int
UseStreptomyces integrating vectorTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-SacB
Plasmid#113635PurposeBroad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacBDepositorInsertsacB
UseTagsExpressionBacterialMutationPlease see Depositor CommentsPromotersacB promoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
LD-RA
Plasmid#209863PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddRFP A
UseTagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDAS12124_PEAR-GFP-preedited
Plasmid#177179PurposePEAR-GFP control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only