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pLKO.1-shRBFOX1-3261

Plasmid

#115458

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shRBFOX1-3261 (RBFOX1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shRBFOX1-3260

Plasmid

#115457

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shRBFOX1-3260 (RBFOX1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-YOD1-C160S

Plasmid

#85666

Purpose

Expression of human YOD1 C160S mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

C160S

Promoter

CMV

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DDX54

Plasmid

#97060

Purpose

Expresses FLAG/HA-tagged DDX54 in mammalian cells

Depositor

Markus Landthaler

Article

Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

Insert

DDX54 (DDX54 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only
plenti-UbcP-HA-YWAA-pGK-HYG

Plasmid

#107379

Purpose

Lentiviral expression of HA-YWAA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

CRBN-Y383A/W385A

Promoter

UbcP

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI

Plasmid

#140202

Purpose

CRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

mCherry

Use

CRISPR and Synthetic Biology; Proof-of-concept te…

Tags

Expression

Mutation

G174D

Promoter

PelcA

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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hEBI3 pEF6-V5 (MJS_002)

Plasmid

#72490

Purpose

Expression of human EBI3 in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

Insert

EBI3 (EBI3 Human)

Use

Tags

His/V5

Expression

Mammalian

Mutation

Promoter

EF-1 alpha

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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prk5-TMEM192-3xHA PM mutant

Plasmid

#149506

Purpose

Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

TMEM192 (TMEM192 Human)

Use

Transient expression

Tags

3XHA

Expression

Mammalian

Mutation

p.LL23-24AA, p.LL28-29AA

Promoter

CMV

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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A*33:01:01

Plasmid

#203288

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*33:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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sh-SRF

Plasmid

#100797

Purpose

expression of shRNA targeting SRF

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

Insert

rat SRF

Use

RNAi

Tags

Expression

Mutation

Promoter

H1

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ239

Plasmid

#108859

Purpose

FnCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

Cpf1 is rice codon optimized

Promoter

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-EGFP S306A

Plasmid

#14122

Depositor

L. Miguel Martins

Article

Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S306A

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
Brg1 Del4 pET28C

Plasmid

#122118

Purpose

Expression Brg1 amino acids 1446-1647 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Use

Tags

His-T7

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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Brg1 Del3 pET28a

Plasmid

#122117

Purpose

Expression Brg1 amino acids 836-1446 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Use

Tags

His-T7

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMC060-HisMS2_PLP_Env_pac

Plasmid

#155040

Purpose

Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope Gene

Depositor

Paul Freemont

Article

Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

Inserts

Maturation Protein
Coat Protein Dimer
Envelope Gene

Use

Tags

Expression

Bacterial

Mutation

Remove TypeIIs Restriction Sites

Promoter

T7

Available since

Sept. 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFRT_TO_FlagHA_CELF1

Plasmid

#106099

Purpose

Inducible gene expression of FlagHA - CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

CELF1 (CELF1 Human)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TRAIL-R3

Plasmid

#61384

Purpose

Expresses TRAIL receptor 3

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 3 (TNFRSF10C Human)

Use

Tags

HA tag and TNF receptor leader peptide

Expression

Mammalian

Mutation

TRAIL-R3 leader peptide (aa1-29) replaced with TN…

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pETM33_EPS15_EH

Plasmid

#178475

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

EPS15_EH (EPS15 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pJ128

Plasmid

#19962

Depositor

Jonathan Tait

Article

Jin et al J Biol Chem. 2004 Sep 24. 279(39):40351-

Insert

annexin V-128 (ANXA5 Human)

Use

Tags

(Met)-Ala-Gly-Gly-Cys-Gly-His

Expression

Bacterial

Mutation

Deletion of the initiator Met at position 1 of wi…

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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pENTR221-CCL5.Pro

Plasmid

#79492

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CCL5 Promoter (CCL5 Human, Synthetic)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

CCL5 Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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