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Plasmid#107174Purposeexpresses V5-tagged miniTurbo on the mammalian ER membraneDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionMammalianMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1deltaTMD
Plasmid#108132PurposeRecruitable cytosolic SAC1DepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-521)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM114
Plasmid#127211PurposeBeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDSDepositorInsertWUS2, IPT, sgRNA, Luc
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM134
Plasmid#127215PurposeBeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDSDepositorInsertLuc, IPT, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pc DNA FRT TO- APP695
Plasmid#114193PurposeCreation of a stable cell line expressing APP mutation (AD in vitro study)DepositorInsertAmyloid-Precursor-Protein (APP Human)
UseTagsExpressionMammalianMutationwild type splice variant 695: 695AA (2085bp, lack…PromoterCMVAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PM-GA
Plasmid#209877PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddGFP A
UseTagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-miniTurbo-V5_pLX304
Plasmid#107176Purposeexpresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vectorDepositorInsertminiTurbo (BirA mutant)
UseLentiviralTagsExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag
Plasmid#209726PurposeExpresses cytosolic medium affinity ratiometric ATP sensorDepositorInsertplenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGem-PgpdA-Cre-TtrpC
Plasmid#168784PurposeHelper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.DepositorInsertCre
UseCre/Lox and Synthetic Biology; Expression in fila…TagsExpressionMutationPromoterPgpdAAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro cPLA2-EGFP
Plasmid#162568PurposeUsed as a measure of cPLA2 activation in live cells with an EGFP tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-b2AR-eLOV-TEVcs-FLAG-GAL4-V5
Plasmid#104841Purposeb2AR-SPARK TF componentDepositorInsertb2AR-eLOV-TEVcs-FLAG-GAL4-V5
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDawn-sfGFP
Plasmid#107741PurposeExpresses superfolder GFP under the control of a blue-light transcriptional regulatorDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterpLambdaAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
FSW-HRP-TM
Plasmid#82540PurposeLentiviral vector for expressing HRP-TM in neuronsDepositorInsertHRP fused to TM domain of PDGFR
UseLentiviralTagsHRPExpressionMammalianMutationPromoterHuman SynapsinAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
LOV-Turbo-His6_pET21a
Plasmid#199668Purposeexpresses LOV-Turbo in bacteriaDepositorInsertLOV-Turbo
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hfYFP
Plasmid#186523PurposeHyperfolder YFP fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertHyperfolder YFP
UseTagsExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRN227
Plasmid#127222PurposeBeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDSDepositorInsertCas9, WUS2, AtSTM, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NAD-Snifit-cyto
Plasmid#113914PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorInsertNAD-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
epi-ABEmax-NG
Plasmid#135976PurposeExpresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication originDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTC217
Plasmid#70018PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1bDepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro dr_cPLA2-mKate2
Plasmid#163844PurposeUsed as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only