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Plasmid#184908Purposecarrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferriduransDepositorInsertCas9-sgRNA-homology arms
UseCRISPR; Broad-host-rangeTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-C
Plasmid#30224DepositorInsertrhVitronectin with C terminus cleavage (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 399-478PromoterAvailable sinceMay 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-tRNA2.0
Plasmid#158393PurposeGolden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
-5.5kb-UCP1-GFP
Plasmid#104585PurposeReporter assay to monitor the transcription activity of the UCP1 promoter by GFP expressionDepositorInsertUCP1 promoter (Ucp1 Mouse)
UseTagsTurboGFPExpressionMammalianMutationPromoterUCP1Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Neo
Plasmid#85139Purposelentiviral backboneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_human_leucinezipperTRAIL _IRES_mCherry_PGK_tBFP
Plasmid#85433PurposeLentiviral vector for Gal4 inducible leucinezipper TRAIL with mCherry reporter and constitutive tBFP expressionDepositorInsertleucine zipper human TRAIL secreted form
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCasAb-apr
Plasmid#121998PurposeBacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumanniiDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PE-bacteria
Plasmid#172715PurposeExpression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editingDepositorInsertCas9n-linker-M-MLV2
UseTagsExpressionBacterialMutationH840A of Cas9PromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
Plasmid#222994PurposeMammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNADepositorInsertHuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
UseCRISPRTagsPuroExpressionMammalianMutationPromoterpCAGAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pdCas9
Plasmid#46569PurposeExpresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.DepositorInsertstracrRNA
dcas9
CRISPR array
UseCRISPRTagsExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
plRL2
Plasmid#163631PurposeOptimized M. tuberculosis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo Fluc EGFP
Plasmid#90170PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteinsDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB-BbsI-sgRNA
Plasmid#203359PurposeContains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backboneDepositorInsertBbsI Cut Sites
UseTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-ZmBbm-P2A-ZmWus2-9
Plasmid#197750PurposePhytobrick (MoClo) Level 0 PartDepositorInsertZmBbm-P2A-ZmWus2 bicistronic CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q, R261Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_ATP1.0
Plasmid#167577PurposeExpress the ATP sensor GRAB_ATP1.0 in neuronsDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEos4b-C1
Plasmid#54812PurposeLocalization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581DepositorTypeEmpty backboneUseTagsmEos4bExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTetR-LasR-pLuxR-GFP
Plasmid#171159PurposeSensing Device with Reporter proteinDepositorInsertslasR
gfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLux and pTetAvailable sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDA_887
Plasmid#201164PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only