-
Plasmid#81064PurposeGateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC (UBR5 Human)
UseCloningTagsExpressionMutationY2402A and K2415A (bp t7204g, a7205c, t7206c, a72…PromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W
Plasmid#163169PurposeLentiviral plasmid with non-targeting gRNA, co-expression of BFP tagDepositorInsertNon-targeting
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DICER-Prom
Plasmid#25851DepositorInsertDicer promoter (DICER1 Human, gene ID 23405)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-CtIP-siR-WT
Plasmid#82030PurposePlasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertCtIP (RBBP8 Human)
UseTagsHAExpressionMammalianMutationMutations making the cDNA resistant to a siRNAPromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/RFP-DDX54
Plasmid#97061PurposeExpresses RFP (tdTomato)-tagged DDX54 in mammalian cellsDepositorInsertDDX54 (DDX54 Human)
UseTagsRFP (tdTomato)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1C
Plasmid#196685PurposeRep/Cap plasmid for the production of PAL1C, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationPTQGTLR insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-myr-CSK-GFP
Plasmid#83470PurposeLentiviral vector for doxycycline-inducible expression of membrane targeted-CSK in mammalian cellsDepositorInsertCSK (CSK Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterdoxycycline-inducible promoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCrysβ:ECFP-LexOP:mCherry-KrasG12V
Plasmid#89888PurposeHuman KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.DepositorInsertmCherry-KRasG12V
UseUnspecifiedTagsmCherryExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
AfrLEA3m_mCh-2xFKBP (pBS1137)
Plasmid#185321PurposeFor the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertAfrLEA3m
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_APOE_HUMAN_E4_47-300 (pBS0847)
Plasmid#185279PurposeFor the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertAPOE_HUMAN_E4_47-300
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINT-PuroDDHNF4a2
Plasmid#31443DepositorInsertHNF4A (HNF4A Human)
UsePhic31/attbTagsFKBP L106PExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
hCEP120-Myc
Plasmid#50381PurposeTet-On inducible expression of Myc-tagged hCEP120DepositorInsertHuman CEP120 (CEP120 Human)
UseTagsMycExpressionMammalianMutationThis hCEP120 contains 4 SNPs (rs6876883, rs659544…PromoterCMV-2xTetOAvailable sinceJuly 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM33_MDM2_SWIB
Plasmid#178481PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertMDM2_SWIB (MDM2 Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcsII-MECL1-flag
Plasmid#86774PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertimmunoproteasome beta subunit 2 (MECL1) (PSMB10 Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterunknownAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ULK2
Plasmid#97075PurposeExpresses ULK2 in mammalian cellsDepositorInsertULK2 (ULK2 Human)
UseTagsFLAGExpressionMammalianMutationnonePromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag
Plasmid#187994PurposeExpresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cellsDepositorInsertmucin2 D1D2D'D3 (MUC2 Human)
UseTags6xHistadine followed by TEV cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-800)-Strep
Plasmid#141344PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-800)-Strep
UseTagsGB1-His6-TEV and Strep-tag (WSHPQFEK)ExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV neo KD Akt1
Plasmid#14550DepositorInsertAkt1 KD (Akt1 Mouse)
UseRetroviralTagsHA tagExpressionMammalianMutationKinase Dead Mutant: K179MPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS
Plasmid#117384PurposeAn AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectivelyDepositorInsertGCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del2 pET28a
Plasmid#122116PurposeExpression Brg1 amino acids 1-837 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTags6XHis and His-T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only