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Showing: 10481 - 10500 of 167150 results

  7. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid
    #107055
    Purpose
    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107055-plasmid-map-sequence-id-208591

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  8. gGBEv6.3-SpCas9

    Plasmid
    #202629
    Purpose
    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.
    Insert
    nSpCas9(D10A)-MPGv6.3
    Expression
    Mammalian
    Mutation
    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…
    Promoter
    hU6, EF1a, CBh
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202629-plasmid-map-sequence-id-398425

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  9. pJRH029

    Plasmid
    #171060
    Purpose
    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Insert
    Gag-spyCas9
    Use
    CRISPR and Lentiviral
    Tags
    2x SV40 NLS and 3x FLAG tag
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171060-plasmid-map-sequence-id-457342

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  10. TRE reporter backbone

    Plasmid
    #158678
    Purpose
    This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry
    Depositor
    Ravid Straussman
    Article
    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    mStrawberry
    Expression
    Mammalian
    Available Since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    158678-plasmid-map-sequence-id-309765

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  13. pV1393

    Plasmid
    #111430
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111430-plasmid-map-sequence-id-212561

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  14. TFORF2216

    Plasmid
    #142925
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NOTO (NOTO Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142925-plasmid-map-sequence-id-397298

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Showing: 10481 - 10500 of 167150 results