A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs.
Maxwell CS, Jacobsen T, Marshall R, Noireaux V, Beisel CL
Methods. 2018 Jul 1;143:48-57. doi: 10.1016/j.ymeth.2018.02.016. Epub 2018 Feb 24.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
105683 | p70a-T7RNAP | Expresses T7 polymerase in cell-free TXTL. |
105684 | CSM160 | Plasmid containing an RFP dropout used to construct randomized PAM library |