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A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs.

Maxwell CS, Jacobsen T, Marshall R, Noireaux V, Beisel CL
Methods. 2018 Jul 1;143:48-57. doi: 10.1016/j.ymeth.2018.02.016. Epub 2018 Feb 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
105683p70a-T7RNAPExpresses T7 polymerase in cell-free TXTL.
105684CSM160Plasmid containing an RFP dropout used to construct randomized PAM library

Antibodies from Article