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Plasmid#106473PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coliDepositorInsertARG1
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO SwiChRca-TS-EYFP
Plasmid#55631PurposeCre-Dependent Chloride ChannelDepositorInsertiC1C2-TS-EYFP
UseAAV and Cre/LoxTagsEYFPExpressionMammalianMutationC128APromoterEf1aAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pS3
Plasmid#50596PurposeBackbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5DepositorInsertsaroE
aroD
aroB
aroG*
ppsA
tktA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmcs
Plasmid#25755PurposeEntry vector with TRE-tight promoter followed by multiple cloning site.DepositorTypeEmpty backboneUseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep
Plasmid#173899PurposeThe pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThrDepositorInsertsThrRS(v1.0)
tRNA(v2.0)CUA
pduX
EF-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKM444
Plasmid#108319PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage IntegraseDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTR47m4
Plasmid#104068PurposeLuciferase Reporter for Formaldehyde SensingDepositorInsertsxluxAB
frmR
frm Promoter m4
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutation23r and 25th nucleotides in sequence mutated from…PromoterAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSQT1313
Plasmid#53370Purposemultiplex gRNA expression plasmidDepositorInsertNone
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCBL101.1
Plasmid#199724PurposeA pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.DepositorInsertMultiple Cloning Site, NPTII
UseTagsExpressionPlantMutation"GAGACC" to "GATACC"PromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-mScarlet
Plasmid#140875PurposeMultisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.DepositorInsertmScarlet
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-3xFLAG-HSVTKp-mCherry
Plasmid#182377PurposeDual expression construct encoding 3xFLAG and mCherry from separate promotersDepositorInserts3xFlag
mCherry
UseTagsExpressionMammalianMutationPromoterHSV TK promoter and SV40 promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad-Xis/Int Reset Flipper
Plasmid#38207DepositorInsertsBxb1-Excisionase
Bxb1 Integrase
UseSynthetic Biology; Biobrick cloning vectorTagsExpressionMutationPromoterpBADAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNO286-3
Plasmid#107746PurposeKey genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)DepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationDeleted AA 223 and 512PromoterJ23106 and J23108Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
4xNLS-pMJ915v2-sfGFP
Plasmid#88921PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTiger-OptoSOS
Plasmid#86439PurposeSingle-construct blue light optogenetic Ras activator for use in DrosophilaDepositorInsertOptoSOS
UseTagstagRFPExpressionInsectMutationPromoterGal4-UASAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJAT77
Plasmid#204295PurposepJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLSLGFP.R
Plasmid#51501PurposeSingle component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the repliconDepositorInsertGFP
UsePlant t-dna plasmidTagsExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBTK622
Plasmid#180592Purposetdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1DepositorInsertstdk
kanR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEN_Tmcs
Plasmid#25751PurposeEntry vector with TRE promoter followed by multiple cloning site.DepositorTypeEmpty backboneUseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMAL728
Plasmid#169152PurposeEncodes OptoQ-AMP6DepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationA79QPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only