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Plasmid#201452PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT2_d2EGFP-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT4-d2EGFP-mCherry
Plasmid#201453PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT4_d2EGFP-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT5-d2EGFP-mCherry
Plasmid#201454PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT5_d2EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBA407-Neo-COVGT5-mCherry
Plasmid#201455PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT5_mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-SPASTIN, L177D
Plasmid#180649PurposeLentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.DepositorInsertSPASTIN
UseLentiviralTagsmCherryExpressionMammalianMutationStarts at M87, L177D mutation, has siRNA resistan…PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-THTAP-VPS9D1, residues 1-97
Plasmid#180640PurposeBacterial expression for VPS9D1 MIT domain, residues 1-97. Has N-terminal HIS-GST tag. Internal ID: WISP20-89.DepositorInsertVPS9D1 (C16orf7 Human)
UseTagsHIS-GSTExpressionBacterialMutationResidues 1-97PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-KATNA1
Plasmid#160051PurposeMammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.DepositorInsertKATNA1 (KATNA1 Human)
UseTagsOne-Strep-Flag (OSF)ExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Myc-KATNB1
Plasmid#160054PurposeMammalian expression vector for KATNB1, (Katanin p80) with N-terminal Myc tag. Internal ID: WISP20-16.DepositorInsertKATNB1 (KATNB1 Human)
UseTagsMycExpressionMammalianMutationPromoterpCAGAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNA1 MIT residues 1-79
Plasmid#180614PurposeBacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20.DepositorInsertKATNA1 (KATNA1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-79PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNAL1 MIT residues 1-87
Plasmid#180618PurposeBacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.DepositorInsertKATNAL1 (KATNAL1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-87PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNAL2 residues 1-98
Plasmid#180619PurposeBacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.DepositorInsertKATNAL2 (KATNAL2 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-98PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 RPS6KC1 MIT, residues 235-316
Plasmid#180622PurposeBacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.DepositorInsertRPS6KC1 (RPS6KC1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 235-316PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-NL63-Protein3
Plasmid#168923PurposeGateway-compatible Entry vectorDepositorInsertHCoV-NL63-Protein3 (HCNV63gp3 )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_SARS-CoV-2-Spike
Plasmid#168891PurposeGateway-compatible Entry vectorDepositorInsertSARS-CoV-2-Spike (S )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCENPBR pc861
Plasmid#167569PurposeHuman CENP-B (aa 1-169 containing DNA-binding domain) cloned into pDsRed1-N1 vector (Clontech).DepositorInsertHomo sapiens centromere protein B (CENPB Human)
UseTagsDs-RedExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-6xHis-TEV-ORF9c
Plasmid#201023PurposeBacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertopen reading frame 9c
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W5F
Plasmid#198443PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W5FPromoterT7 promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
5ZiPro
Plasmid#198432PurposeConformation sensitive site specific Cys-labelingDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationS84*C, R95*A, C80S, C143S, S160C, C178S,PromoterT7 promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W61*F
Plasmid#198442PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW61*F, R95*A,PromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro W5F
Plasmid#198436PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW5FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only