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Plasmid#58782PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330S-7
Plasmid#58783PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-dCas9-10XGP41-J9
Plasmid#202005PurposeMoClo level 0 dCas9-10XGP41 coding sequence moduleDepositorInsertdCas9-10XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersExpressionMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSR43.6r
Plasmid#63197PurposeExpresses CcaS, Ho1, and PcyA constitutivelyDepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-D156R (ttLbCas12a)
Plasmid#195366PurposeA temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12aDepositorInsertLbCas12a-D156R (ttLbCas12a)
UseCRISPR; Gateway compatible lbcas12a-d156r (ttlbca…TagsExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGE161
Plasmid#79453Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, nptIIDepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFW11-ffluc
Plasmid#128487PurposeAllelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imagingDepositorInsertfirefly luciferase gene
UseAllelic replacement vectorTagsspy0535 downstream flanking region and spy0535 up…ExpressionMutationPromotersyntheticAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW199b2
Plasmid#136928PurposeDirected evolution of SpCas9DepositorInsertgIII
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAT62
Plasmid#204301PurposeExpress phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.DepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLys-M1
Plasmid#109382PurposeArabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coliDepositorInsertpARABAD-VioB-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B4
Plasmid#31021DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBT138b-proD
Plasmid#138516PurposeP3eDepositorInsertT7-RNAP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-TV
Plasmid#158409PurposeCRISPR-dpcoCas9 fused with TV activator for transcriptional activationDepositorInsertdpcoCas9-TV(6xTAL-2xVP64)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTarget12f1
Plasmid#171183PurposeConstitutive expression of sgRNA without donor DNADepositorInsertsgRNA scaffold in the CRISPR/Cas12f1 system
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119(SpeI) promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B2
Plasmid#31019DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMK782 Septin6-GFP
Plasmid#38296DepositorInsertSeptin 6 (Septin6 Mouse)
UseTagseGFPExpressionMammalianMutation22 amino acid linker sequence (added by PCR)PromoterCMVAvailable sinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pC1-CMV-DAAO-NES
Plasmid#141107PurposeExpresses DAAO with nuclear export signal in mammalian cellsDepositorInsertDAAO-NES
UseTagsNuclear export signalExpressionMammalianMutationdeleted DAAO amino acids 367-368PromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCBL101.1
Plasmid#199724PurposeA pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.DepositorInsertMultiple Cloning Site, NPTII
UseTagsExpressionPlantMutation"GAGACC" to "GATACC"PromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B5
Plasmid#31022DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B6
Plasmid#31023DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only