-
Plasmid#127098PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system.DepositorInsertCas9-2A-eGFP
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
XLone-PE2
Plasmid#136463PurposeExpresses prime editing 2 (PE2) enzyme upon doxycycline treatmentDepositorInsertPE2
UseTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pKSB- 3xP3-mTurqSV40 GGGG AAGA
Plasmid#174533Purpose3xP3-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-mTurquoise BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHTganA-Cpf1
Plasmid#158647PurposeConstitutive transcription of FnCpf1 and ganA crRNADepositorInsertCpf1, ganA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baciā¦TagsExpressionBacterialMutationPromoterP43Available sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pADsacA
Plasmid#158649PurposeConstitutive transcription of sacA crRNA and GFPDepositorInsertsacA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baciā¦TagsExpressionBacterialMutationPromoterPvegAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-GFPsv40 GGGG AAGA
Plasmid#174531Purpose3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-GFP BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pKSB- 3xP3-YFPsv40 GGGG AAGA
Plasmid#174530Purpose3xP3-YFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-YFP BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-CAG-hCAS9-neo
Plasmid#166026PurposeAAVS1 targeting vector carrying CAG-SpCas9-neoDepositorInsertAAVS1-CAGCas9IRESneo
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSLBP_mNG_FS_Donor
Plasmid#191387PurposeDonor for C-terminal tagging of endogenous hSLBPDepositorInsertmNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus (SLBP Synthetic, Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLinker-6xHA-HXGPRT-LoxP
Plasmid#86552PurposeThe plasmid is used for endogenous tagging using CRISPR technology in ToxoplasmaDepositorInsertLinker-6xHA-HGPRT-LoxP
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
HCP5
Plasmid#166107PurposeThis plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.DepositorInsertCyr1CT-sgRNA (CYR1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>GAL80
Plasmid#104874PurposeHACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.DepositorInsertT2A-GAL80
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUA97
Plasmid#69340PurposeIt encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategyDepositorInsertceBFP SEC 3xFlag
UseTags3xFlag and ceBFP (mTagBFP)ExpressionWormMutationsqt-1(e1350)PromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAct-Fok1:dCas9
Plasmid#62211PurposeExpresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.DepositorInsertFok1-dCas9
UseTagsExpressionInsectMutationPromoteract5CAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
TLCV2 sgAAVS1
Plasmid#127099PurposeEncodes gRNA for human AAVS1.DepositorInsertAAVS1 (PPP1R12C AAVS1 Human) (PPP1R12C Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBsacA
Plasmid#158650PurposeConstitutive transcription of sacA crRNADepositorInsertsacA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baciā¦TagsExpressionBacterialMutationPromoterPvegAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDonorTp-fugfp
Plasmid#211790PurposepDonorTp with addition of fugfp gene from pUS252 outside of the FRT sitesDepositorInsertfuGFP
UseTagsExpressionMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
hPLD3-sgRNA-Cas9_mcherry
Plasmid#199342Purposeencodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherryDepositorInserthSpCas9
UseCRISPRTagsP2A-RFPExpressionMammalianMutationPromoterhuman U6Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only