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pCAGGS-3xFLAG-Rbfox2

Plasmid

#213726

Purpose

Mammalian cell expression vector for overexpression of Rbfox2 in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

Rbfox2 (Rbfox2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRCH-SsAPOBEC3B

Plasmid

#198550

Purpose

Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH-SsAPOBEC3B

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pNS20-SpCas9-SNAP

Plasmid

#113717

Purpose

Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

Depositor

Gerald Schwank

Article

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

Cas9

Use

CRISPR

Tags

HA-2xNLS-SNAP-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-KATNA1

Plasmid

#160051

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Use

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-ChroME-mRuby2-ST

Plasmid

#108902

Purpose

Cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChroME-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas9-162A

Plasmid

#84918

Purpose

for in vitro transcription of Cas9 with polyA tail encoded

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Cas9

Use

Tags

Flag, NLS, and polyA tail

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide B

Plasmid

#194011

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide B)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide A

Plasmid

#194008

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide A)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide B

Plasmid

#194009

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide B)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide A

Plasmid

#194010

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide A)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

Plasmid

#86612

Purpose

Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR; Co-selection via nhej using ouabain

Tags

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCasper_Pdf-LexA::VP16

Plasmid

#33259

Depositor

Michael Rosbash

Article

Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;10

Insert

LexA::VP16-SV40 3'UTR (lexA E. coli)

Use

Tags

Expression

Insect

Mutation

Promoter

Pdf

Available since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only
G1397 DddAtox-N–dSpCas9

Plasmid

#157835

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3CC-FLAG

Plasmid

#179162

Purpose

Expression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

Insert

protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CD4-myc

Plasmid

#58537

Purpose

Expresses human CD4 with a C-terminal myc tag in mammalian cells

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

CD4 (CD4 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pSpCas9(BB)-T2A-HygR

Plasmid

#118153

Purpose

SpCas9 expression vector

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

hSpCas9-T2A-HygR

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEa-V106W

Plasmid

#193838

Purpose

Express TadCBEa-V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDa V106W-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-Rbfox3

Plasmid

#213727

Purpose

Mammalian cell expression vector for overexpression of Rbfox3 in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

Rbfox3 (Rbfox3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFREE2-SpCas9-sgRNA

Plasmid

#179582

Purpose

Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

Depositor

Kevin Solomon

Article

Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

Insert

Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-HiBiT-CD9

Plasmid

#162592

Purpose

N-terminally HiBiT-tagged human CD9 under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

human CD9 (CD9 Synthetic, Human, human)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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