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Plasmid#72234PurposeUracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoterDepositorInsertPP7-PS-2x-yeGFP
UseTagsExpressionYeastMutationPromoterADE3pAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
GPKAnes
Plasmid#61092PurposeGFP-tagged PKA catalytic subunit targeted to the extranuclear cytoplasm by addition of a nuclear export signalDepositorInsertpkac2
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-R1868*-3xFLAG
Plasmid#130679PurposeExpresses human SMCHD1 R1868* with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 R1868* (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationR1868_V2005delinsXPromoterCMVAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon37-48-3xFLAG
Plasmid#130688PurposeExpresses human SMCHD1 Exon37-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon37-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutation1M_T1522delinsMPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-36-3xFLAG
Plasmid#130681PurposeExpresses human SMCHD1 Exon1-36 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-36 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationD1523_V2005delinsXPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago Chi-a
Plasmid#14075DepositorInsertAgo Chi-a
UseTagsFLAG/HAExpressionMammalianMutationAgo Chimera-aPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDZ529 pTRP MET25p PP7-PS-2x-yeGFP
Plasmid#72233PurposeTryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoterDepositorInsertPP7-PS-2x-yeGFP
UseTagsExpressionYeastMutationPromoterMET25Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago Chi-b
Plasmid#14085DepositorInsertAgo Chi-b
UseTagsFLAG/HAExpressionMammalianMutationAgo Chimera-bPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago Chi-c
Plasmid#14086DepositorInsertAgo Chi-c
UseTagsFLAG/HAExpressionMammalianMutationAgo Chimera-cPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago Chi-d
Plasmid#14087DepositorInsertAgo Chi-d
UseTagsFLAG/HAExpressionMammalianMutationAgo Chimera-dPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseA-6xHis
Plasmid#214808PurposeExpression of 6xhis-tagged micrococcal nuclease A (MNaseA)DepositorInsertOmpA-Micrococcal nuclease A
UseTags6xhisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseB-6xHis
Plasmid#214809PurposeExpression of 6xhis-tagged micrococcal nuclease B (MNaseB)DepositorInsertOmpA-Micrococcal nuclease B
UseTags6xhisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2696-CMV-Nme2SmuCas9-D56R
Plasmid#228717PurposeExpresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cellsDepositorInsertNme2SmuCas9-D56R nuclease
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2697-CMV-Nme2SmuCas9-E932R
Plasmid#228718PurposeExpresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cellsDepositorInsertNme2SmuCas9-E932R nuclease
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1177
Plasmid#217961PurposeDual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.DepositorInsertHistone H3 (HIST2H3PS2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSmg5-siRNAres-V5His6_F
Plasmid#146291PurposeInsect Expression of DmSmg5-siRNAresDepositorInsertDmSmg5-siRNAres (Smg5 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSMG5-PINSMG6-V5His6_E
Plasmid#146232PurposeInsect Expression of DmSMG5-PINSMG6DepositorInsertDmSMG5-PINSMG6 (Smg5 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSMG5-siRNAres-V5His6_E
Plasmid#146233PurposeInsect Expression of DmSMG5-siRNAresDepositorInsertDmSMG5-siRNAres (Smg5 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSMG6-dellinker-siRNAres-V5His6_E
Plasmid#146234PurposeInsect Expression of DmSMG6-dellinker-siRNAresDepositorInsertDmSMG6-dellinker-siRNAres (Smg6 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSMG6-delPIN-siRNAres-V5His6_E
Plasmid#146235PurposeInsect Expression of DmSMG6-delPIN-siRNAresDepositorInsertDmSMG6-delPIN-siRNAres (Smg6 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only