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Plasmid#195537PurposedCas9 effector fusion for manipulating transcriptionDepositorArticleInsertLenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT342 Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
Plasmid#195541PurposedCas9 effector fusion for manipulating transcriptionDepositorArticleInsertLenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-SF14P
Plasmid#209448Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from SF14P promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-kasOP*
Plasmid#209447Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from kasOP* promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-Luciferase-CD4-bla
Plasmid#189981PurposeDonor Vector to integrate firefly Luciferase C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertLuciferase
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-Halo-CD4-bla
Plasmid#189982PurposeDonor Vector to integrate HaloTag C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertHaloTag
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hCRY2-mScarlet-I-CD4-bla
Plasmid#189984PurposeDonor Vector to integrate mScarlet-I C-terminal into the human CRY2 Locus to express it as a fusion proteinDepositorInsertmScarlet-I
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hCRY2-Luciferase-CD4-bla
Plasmid#189985PurposeDonor Vector to integrate firefly Luciferase C-terminal into the human CRY2 Locus to express it as a fusion proteinDepositorInsertLuciferase
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hCRY2-Halo-CD4-bla
Plasmid#189986PurposeDonor Vector to integrate HaloTag C-terminal into the human CRY2 Locus to express it as a fusion proteinDepositorInsertHaloTag
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCriprV2-sgRNA-PER1-#2
Plasmid#189988PurposeLentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982DepositorInsertPeriod1 (PER1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer20-iCreb5(HA)
Plasmid#195023PurposeHA-tagged Creb5 in pInducer20 lentivirus destination vectorDepositorInsertHA-tagged Creb5Â (full length, 508aa) (CREB5 Bovine)
UseLentiviralTags3xHAExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 128)
Plasmid#195031PurposeGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa)DepositorInsertGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa) (CREB5 Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(G137I)
Plasmid#133272PurposeX. laevis expression vector. It will generate the mouse TREK-1 channelDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
UseOocyte expressionTagsExpressionMutationG137IPromoterT7Available sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-NRF1-V5-miniTurbo
Plasmid#207810PurposeC-terminal tagged NRF1 for miniTurbo proximity experimentsDepositorInsertNRF1-V5-miniTurbo (NFE2L1 Human)
UseLentiviralTagsV5-miniTurboExpressionMutationPromoterUBCAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-NRF1
Plasmid#207809PurposeN-terminal tagged NRF1 for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-NRF1 (NFE2L1 Human)
UseLentiviralTagsminiTurbo-V5ExpressionMutationPromoterUBCAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RUNX3(13))-PGKpuro2ABFP-W
Plasmid#208552PurposeLentiviral vector expressing gRNA targeting human RUNX3DepositorInsertRUNX3(13) (RUNX3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RUNX3(12))-PGKpuro2ABFP-W
Plasmid#208551PurposeLentiviral vector expressing gRNA targeting human RUNX3DepositorInsertRUNX3(12) (RUNX3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Control(1))-PGKpuro2AmCherry-W
Plasmid#210612PurposeLentiviral vector expressing gRNA targeting a control region at the CXCR4 locusDepositorInsertControl(1)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RIC8A(16))-PGKpuro2ABFP-W
Plasmid#200491PurposeLentiviral vector expressing gRNA targeting human RIC8ADepositorInsertRIC8A(16) (RIC8A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RIC8A(15))-PGKpuro2ABFP-W
Plasmid#200490PurposeLentiviral vector expressing gRNA targeting human RIC8ADepositorInsertRIC8A(15) (RIC8A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only