pGEMHE:mTREK-1(G137I)
(Plasmid
#133272)
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PurposeX. laevis expression vector. It will generate the mouse TREK-1 channel
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133272 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEMHE
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Vector typeOocyte Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePotassium channel subfamily K member 2 (KCNK2, TREK-1)
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SpeciesM. musculus (mouse)
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MutationG137I
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Entrez GeneKcnk2 (a.k.a. A430027H14Rik, TREK-1)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEMHE:mTREK-1(G137I) was a gift from Dan Minor (Addgene plasmid # 133272 ; http://n2t.net/addgene:133272 ; RRID:Addgene_133272) -
For your References section:
K2P2.1 (TREK-1)-activator complexes reveal a cryptic selectivity filter binding site. Lolicato M, Arrigoni C, Mori T, Sekioka Y, Bryant C, Clark KA, Minor DL Jr. Nature. 2017 Jul 20;547(7663):364-368. doi: 10.1038/nature22988. Epub 2017 Jul 10. 10.1038/nature22988 PubMed 28693035
