Showing: 10281 - 10300 of 18099 results
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Plasmid#61357Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (D1399Y)
Plasmid#61358Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; D1399Y mutation i…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-Nm-dCas9-p300 Core
Plasmid#61365Purposeencodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertNm-dCas9-p300 Core (EP300 Human, Synthetic, Neisseria meningiditis)
UseCRISPR and Synthetic BiologyTagsHA tag and NLSExpressionMammalianMutationD16A, D587A, H588A, N611A in Nm Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pZDonor-Nm-Cas9-gRNA-hU6
Plasmid#61366Purposeempty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNADepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6AvailabilityAcademic Institutions and Nonprofits only -
p5E-4xnrUAS (L4-R1)
Plasmid#61372Purpose5' Gateway Entry Clone Containing 4xnrUASDepositorInsert4xnrUAS
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
4xnrUAS:GFP
Plasmid#61373Purpose4xnrUAS driving GFPDepositorInsert4xnr UAS:GFP
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p5E-QUAS (L4-R1)
Plasmid#61374Purpose5' Gateway Entry Vector Containing QUASDepositorInsertQUAS
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TRAIL-R1
Plasmid#61382PurposeExpresses HA tagged TRAIL receptor 1DepositorInsertTRAIL receptor 1
UseTagsHA and TNF receptor leader peptideExpressionMammalianMutationTRAIL-R1 leader peptide (aa1-23) replaced with TN…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TRAIL-R2
Plasmid#61383PurposeExpresses TRAIL receptor 2DepositorInsertTRAIL receptor 2 (TNFRSF10B Human)
UseTagsnoneExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRIP3-mCherry
Plasmid#61386PurposeExpresses RIP3-mCherry in mammalian cellsDepositorInsertRIP3 (RIPK3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCS2-Tol1.zf1
Plasmid#61388PurposemRNA synthesis of tol1 transposase codon modified for zebrafishDepositorInserttol1.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6AvailabilityAcademic Institutions and Nonprofits only -
pCS2-TagRFPT.zf1
Plasmid#61390PurposemRNA synthesis of TagRFPT codon modified for zebrafishDepositorInsertTagRFPT.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cre.zf1
Plasmid#61391PurposemRNA synthesis of Cre recombinase codon modified for zebrafishDepositorInsertCre.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Gal4ff.zf1
Plasmid#61392PurposemRNA synthesis of Gal4ff codon modified for zebrafishDepositorInsertGal4ff.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6AvailabilityAcademic Institutions and Nonprofits only -
pL-SFFV.Reporter.RFP657.PAC
Plasmid#61395PurposeLentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pNeuroD-ires-GFP
Plasmid#61403PurposeMammalian expression vector under the neuron-specific promoter NeuroD. Co-expression of GFP with IRES sequenceDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterNeuroDAvailabilityAcademic Institutions and Nonprofits only -
pCITF-KCC2
Plasmid#61404PurposeMammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequenceDepositorInsertKCC2 (SLC12A5 Human)
UseTagsIRES-tdTomato-FlagExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (full-length, aa 1-481)
Plasmid#61405PurposeExpresses human OTUD1 (full-length) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
LSL-Cas9-Rosa26TV
Plasmid#61408PurposeCre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.DepositorInsertsCas9
EGFP
UseCRISPR, Cre/Lox, and Mouse TargetingTags3xFLAG and P2AExpressionMammalianMutationhuman codon optimizedPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD2 (full-length, aa 1-348)
Plasmid#61409PurposeExpresses human OTUD2 (full-length) in E. coli.DepositorInsertOTUD2 (YOD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 10281 - 10300 of 18099 results