-
Plasmid#101300PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-509, 670-698, and 699-906 …PromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_KPNA4_Arm
Plasmid#178495PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertKPNA4_Arm (KPNA4 Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-E-cadherin promoter
Plasmid#61798PurposeLuciferase reporter containing the mouse E-cadherin promoterDepositorInsert-178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
hWIP1 D314A FLAG
Plasmid#28106DepositorInsertWIP1 (PPM1D Human)
UseTagsFLAGExpressionMammalianMutationContains phosphatase-dead mutation D314APromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
Myc-Foxo1 D256
Plasmid#12145DepositorInsertFoxo1 (Foxo1 Mouse)
UseTagsMycExpressionMammalianMutationContains a deletion truncating the protein after …PromoterCMVAvailable sinceAug. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Flag-hKlf4
Plasmid#20074DepositorInserthKlf4 (KLF4 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Flag-PGC1a-6His
Plasmid#67637PurposeAAV vector expressing PGC1a geneDepositorInsertPGC1a (Ppargc1a Mouse)
UseAAVTags6XHis and Flag tagExpressionMutationPromoterCMVAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4_ZV-NS5-RdRp
Plasmid#179847PurposeThe RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89DepositorInsertZika Virus Non-structural protein 5 RdRp domain
UseTags6-histineExpressionBacterialMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuroN3xFLAG-His-CDK5RAP3
Plasmid#204555PurposeExpresses 3xFLAG-His-tagged CDK5RAP3 in mammalian cellsDepositorInsertCDK5RAP3 (AMY2A Human)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHalo-MyosinIIA-C25
Plasmid#128573PurposeStudies of cellular contractilityDepositorInsertMyosin IIA (Myh9 Mouse)
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW_TOPO_Cdk12_Untagged
Plasmid#127176PurposeUntagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vectorDepositorInsertMouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UseEntry vector with transgene for gateway cloningTagsNoneExpressionMutationPromoterNoneAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPW3758 : CMVd3-HsIRE1-HaloTag
Plasmid#185680PurposeLow-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_ELAVL1
Plasmid#106106PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL1DepositorInsertgRNA targeting ELAVL1 (ELAVL1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hAtg12
Plasmid#87869PurposeExpress a EGFP-human Atg12/Apg12 in mammalian cellsDepositorInsertATG12 (ATG12 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHS1518
Plasmid#205981PurposeFLAG-SV40 NLS-Cas6(Cse3/CasE) from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas6(Cse3/CasE) from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHS1514
Plasmid#205977PurposeFLAG-SV40 NLS-Cas8/Cse1 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas8/Cse1 from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1515
Plasmid#205978PurposeFLAG-SV40 NLS-Cas11/Cse2 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas11/Cse2 from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-GFP
Plasmid#40592DepositorInsertGFP-S65T
UseMouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionBacterial and MammalianMutationConstruct contains a mutated, humanized hGFP(S65T…PromoterhGFAP promoterAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
integrin alpha9 pcDNA1 neo
Plasmid#13585DepositorInsertintegrin alpha 9 (ITGA9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
GFP-Mfn1-E209A
Plasmid#141155PurposeMammalian expression and labeling of mutant mitofusin 1 (E209A) deficient in GTP hydrolysisDepositorInsertMFN 1 (MFN1 Human)
UseTagsAcGFPExpressionMammalianMutationchanged Glutamic acid at position 209 to Alanine …PromoterCMVAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only