Showing: 10261 - 10280 of 13976 results
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Plasmid#149424PurposeThe piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)DepositorInsertCas9
UseCRISPRTagseGFPExpressionInsectMutationPromoterDmel Hsp70BbAvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4095420
Plasmid#149523PurposeBacterial expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationd1-745 d1063-1945PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4095421
Plasmid#149524PurposeBacterial expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationd1-745 d1063-1945PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4095418
Plasmid#149525PurposeBacterial expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationd1-1088 d1203-1945PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4095419
Plasmid#149526PurposeBacterial expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationd1-1088 d1203-1945PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4136463
Plasmid#149527PurposeBacterial expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…AvailabilityIndustry, Academic Institutions, and Nonprofits -
hPCNA-S228I
Plasmid#190938Purposeuntagged human PCNA with a hypomorphic disease-associated serine to isoleucine mutation at residue 228DepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
UseTagsExpressionBacterialMutationchanged serine 228 to isoleucinePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-H2B mCherry-XL (R125K)
Plasmid#189926PurposeExpresses nuclear-localized mCherry-XL (R125K) in mammalian cellsDepositorInsertmCherry-XL (R125K)
UseTagsH2BExpressionMammalianMutationdiffers from mCherry by R125K, W143S, I161V, Q163…PromoterCMV7AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53-DsbC
Plasmid#186623PurposeE. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.DepositorTypeEmpty backboneUseTagsDsbC-6His-TEVExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28b_RanWT
Plasmid#186270PurposeExpression vector of His-RanQ69L in E.coliDepositorInsertGTP-binding nuclear protein Ran
UseTagsHis taggedExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
cr3Ec
Plasmid#184860Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination systemDepositorInsertCas9, gam, bet, exo
UseTagsCas9 ssrA degradation tagExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
cr3Vn
Plasmid#184861Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination systemDepositorInsertCas9, gam, bet, exo
UseTagsCas9 ssrA degradation tagExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
cr3Dd
Plasmid#184862Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination systemDepositorInsertCas9, bet, exo
UseTagsCas9 ssrA degradation tagExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p3xnls-mScarlet3_N1
Plasmid#189775PurposeConstruct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3DepositorInsert3xnls-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-LaminB_C1
Plasmid#189776PurposeConstruct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusionDepositorInsertmScarlet3-LaminB
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO1(wt)
Plasmid#191798PurposeFull length wt AtPCO1 with Nt-His tagDepositorInsertAtPCO1
UseTags6xHis, Thrombin cleavage siteExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO2(wt)
Plasmid#191799PurposeFull length wt AtPCO2 with Nt-His tagDepositorInsertAtPCO2
UseTags6xHis, Thrombin cleavage siteExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO3(wt)
Plasmid#191800PurposeFull length wt AtPCO3 with Nt-His tagDepositorInsertAtPCO3
UseTags6xHis, Thrombin cleavage siteExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)
Plasmid#191801PurposeFull length wt AtPCO4 with Nt-His tagDepositorInsertAtPCO4-1
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-TEV-AtPCO5(wt)
Plasmid#191803PurposeFull length wt AtPCO5 with Nt-His tagDepositorInsertAtPCO5
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 10261 - 10280 of 13976 results