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pHR-FKBP:mCherry-Rab5a

Plasmid

#72901

Purpose

Early endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab5a (RAB5A Human)

Use

Lentiviral

Tags

FKBP(F36V) and mCherry

Expression

Mammalian

Mutation

fragment a.a. 2-215

Promoter

Availability

Academic Institutions and Nonprofits only

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pHR-FKBP:mCherry-Rab11a

Plasmid

#72902

Purpose

Recycling endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab11a (RAB11A Human)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

fragment a.a. 2-216

Promoter

Availability

Academic Institutions and Nonprofits only

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pHR-FKBP:mCherry-Rab7a

Plasmid

#72903

Purpose

Late endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab7a (RAB7A Human)

Use

Lentiviral

Tags

FKBP, mCherry

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pHR-TcRb:mCherry-FKBP

Plasmid

#72904

Purpose

ER-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

T cell receptor β chain

Use

Lentiviral

Tags

biotinylation acceptor peptide (AP) and mCherry, …

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pHR-FKBP:mCherry-BclXLc33

Plasmid

#72905

Purpose

Outer mitochondrial membrane-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Bl2-like protein 1, isoform Bcl-X(L) (fragment)

Use

Lentiviral

Tags

FKBP, mCherry

Expression

Mammalian

Mutation

33 C-terminal a.a.

Promoter

Availability

Academic Institutions and Nonprofits only

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C1-FKBP(F36V):mCherry-Akt2(K14A)

Plasmid

#72906

Purpose

Recruitable Akt2(K14A) mutant

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

RAC-beta serine/threonine-protein kinase (AKT2 Human)

Use

Tags

FKBP(F36V), mCherry

Expression

Mammalian

Mutation

K14A mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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N1-mSin1.1-GFP

Plasmid

#72907

Purpose

GFP-tagged mSin1 (isoform 1)

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

mSin1.1 (MAPKAP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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N1-mSin1.2-GFP

Plasmid

#72908

Purpose

GFP-tagged mSin1 (isoform 2)

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Target of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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N1-mSin1.5-GFP

Plasmid

#72909

Purpose

GFP-tagged mSin1 (isoform 5)

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Target of rapamycin complex 2 subunit MAPKAP1, isoform 5 (MAPKAP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant

Plasmid

#72922

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA Binding Protein 6 (GATA6 Human)

Use

Entry vector for gateway cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Mutation

Promoter

TRE tight

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

Plasmid

#72924

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA Binding Protein 6 (GATA6 Human)

Use

Entry vector for gateway cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Mutation

Serine#37 changed to Alanine;Silent mutations mad…

Promoter

TRE tight

Availability

Academic Institutions and Nonprofits only

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pCGN H-Ras 61L

Plasmid

#14720

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

H-ras (HRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

61L

Promoter

Availability

Academic Institutions and Nonprofits only
pCGN H-Ras 61L, 189L

Plasmid

#14721

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

H-ras (HRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

61L, 189L

Promoter

Availability

Academic Institutions and Nonprofits only
pCGN K-Ras 12V, 188L

Plasmid

#14722

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

K-ras (KRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

12V, 188L

Promoter

Availability

Academic Institutions and Nonprofits only
pCGN N-Ras wt

Plasmid

#14723

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

N-ras (NRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCGN N-Ras 189L

Plasmid

#14724

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

N-ras (NRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

189L

Promoter

Availability

Academic Institutions and Nonprofits only
pCGN N-Ras 12D

Plasmid

#14725

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

N-ras (NRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

12D

Promoter

Availability

Academic Institutions and Nonprofits only
pCGN N-Ras 12D, 189L

Plasmid

#14726

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

N-ras (NRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

12D, 189L

Promoter

Availability

Academic Institutions and Nonprofits only
pCGN R-Ras wt

Plasmid

#14727

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

R-ras (RRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pCGN R-Ras 38V

Plasmid

#14728

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

R-ras (RRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

38V

Promoter

Availability

Academic Institutions and Nonprofits only