Showing: 10241 - 10260 of 18099 results
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Plasmid#68134PurposeExpression of Cerulean-tagged, kinase-dead Plk1 in mammalian cellsDepositorInsertPolo-like kinase 1 (PLK1 Human)
UseTagsCeruleanExpressionMammalianMutationK82R kinase-dead versionPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pUPD2
Plasmid#68161PurposeUniversal domesticator plasmid for level 0 partsDepositorInsertempty vector
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDGB3alpha2_35S:P19:Tnos (GB1203)
Plasmid#68214PurposeTranscriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoterDepositorInsertP19 silencing suppressor
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:Renilla:Tnos (GB0109)
Plasmid#68215PurposeTranscriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoterDepositorInsertRenilla
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:Luciferase:Tnos (GB0110)
Plasmid#68216PurposeTranscriptional unit for Firefly Luciferase Protein plant expression driven by the 35S promoterDepositorInsertLuc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailabilityAcademic Institutions and Nonprofits only -
pICSL11056
Plasmid#68258PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plantsDepositorInsertPromoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pICSL11059
Plasmid#68263PurposeLevel 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassetteDepositorInsertPromoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HisC EXO1b
Plasmid#68268PurposeMammalian expression of human EXO1DepositorInsertEXO1 (EXO1 Human)
UseTagsHisExpressionMammalianMutationb form, codons 1-846. Please see depositor commen…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
GFP E17TAG
Plasmid#68295PurposeE17TAG GFP reporterDepositorInsertE17TAG GFP
UseTagsHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1 phiC31
Plasmid#68310PurposeIn vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).DepositorInsertphiC31
UseRna transcriptionTagsExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDestattB (pCM268)
Plasmid#68313PurposeMultisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafishDepositorTypeEmpty backboneUseZebrafish transgenesisTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PX404 Campylobacter jejuni Cas9
Plasmid#68338PurposeCampylobacter jejuni Cas9DepositorInsertCjCas9
UseCRISPRTagsHA-NLS and NLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDestattB_ubi:EGFP (pCM272)
Plasmid#68339PurposeMultisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.DepositorInsertEGFP
UseZebrafish transgenesisTagsExpressionMutationPromoterubiAvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-Cas9Bsd-W
Plasmid#68343PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termi…ExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
TriEx-4 DHX36
Plasmid#68368PurposeExpresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.DepositorInsertDHX36 (DHX36 Human)
UseTagsHistidineExpressionBacterial, Insect, and Mamm…MutationPromoterCMV ie enhancer/promoterAvailabilityAcademic Institutions and Nonprofits only -
PGR-Blue
Plasmid#68374PurposeTerminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.DepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assemblyTagsExpressionBacterialMutationPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianMutationPromoterhuman PGK1AvailabilityAcademic Institutions and Nonprofits only -
V96 ELP
Plasmid#68392PurposeElastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residueDepositorInsertV96 ELP
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
S48I48 ELP
Plasmid#68394PurposeElastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residueDepositorInsertS48I48 ELP
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 10241 - 10260 of 18099 results